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Evaluating the Toxicity of Hydroxyapatite Nanoparticles in Catfish Cells and Zebrafish Embryos

Authors

  • Xinxin Zhao,

    1. School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue, 639798, Singapore
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  • Kimberly J. Ong,

    1. Department of Biological Science, University of Alberta, CW 405, Biological Sciences Bldg, Edmonton, AB, Canada, T6G 2E9
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  • James D. Ede,

    1. Department of Biological Science, University of Alberta, CW 405, Biological Sciences Bldg, Edmonton, AB, Canada, T6G 2E9
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  • James L. Stafford,

    1. Department of Biological Science, University of Alberta, CW 405, Biological Sciences Bldg, Edmonton, AB, Canada, T6G 2E9
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  • Kee Woei Ng,

    1. School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue, 639798, Singapore
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  • Greg G. Goss,

    Corresponding author
    1. Department of Biological Science, University of Alberta, CW 405, Biological Sciences Bldg, Edmonton, AB, Canada, T6G 2E9
    • Department of Biological Science, University of Alberta, CW 405, Biological Sciences Bldg, Edmonton, AB, Canada, T6G 2E9
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  • Say Chye Joachim Loo

    Corresponding author
    1. School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue, 639798, Singapore
    • School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue, 639798, Singapore.
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Abstract

The toxicity of needle-(nHA-ND) and rod-shaped (nHA-RD) hydroxyapatite (HA) nanoparticles is evaluated in vitro on catfish B-cells (3B11) and catfish T-cells (28s.3) and in vivo on zebrafish embryos to determine if biological effects are similar to the effects seen in mammalian in vitro systems. Neither nHA-ND nor nHA-RD affect cell viability at concentrations of 10 to 300 μg mL−1. However, 30 μg mL−1 needle-shaped nHA lower metabolic activity of the cells. Axial deformations are seen in zebrafish exposed to 300 μg mL−1 needle shaped nHA after 120 h. For the first time, nHA is reported to cause zebrafish hatching delay. The lowest concentration (3 μg mL−1) of both types of nHA cause the highest hatching inhibition and needle-shaped nHA exposed zebrafish exhibit the lowest hatch at 72 h post fertilization.

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