One Step Synthesis of Gold-Loaded Radial Mesoporous Silica Nanospheres and Supported Lipid Bilayer Functionalization: Towards Bio-Multifunctional Sensors

Authors

  • Remi Veneziano,

    1. Institut Charles Gerhardt Montpellier, Equipe MACS, UMR 5253 CNRS-ENSCM-UM2-UM1, 8 rue de l'Ecole, Normale, 34296 Montpellier Cedex 5, France
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  • Gaelle Derrien,

    1. Institut Charles Gerhardt Montpellier, Equipe Agrégats, Interfaces et, Matériaux pour l'Energie, UMR 5253 CNRS- Université Montpellier 2, C. C. 1502, Place Eugène Bataillon, 34095 Montpellier cedex 5, France
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  • Sisareuth Tan,

    1. Chimie et Biologie des Membranes et des Nanoobjets, Equipe Imagerie Moléculaire et Nanobiotechnologie, UMR 5248 CNRS- Université de Bordeaux, 33402 Talence Cedex, France
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  • Alain Brisson,

    1. Chimie et Biologie des Membranes et des Nanoobjets, Equipe Imagerie Moléculaire et Nanobiotechnologie, UMR 5248 CNRS- Université de Bordeaux, 33402 Talence Cedex, France
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  • Jean-Marie Devoisselle,

    1. Institut Charles Gerhardt Montpellier, Equipe MACS, UMR 5253 CNRS-ENSCM-UM2-UM1, 8 rue de l'Ecole, Normale, 34296 Montpellier Cedex 5, France
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  • Joel Chopineau,

    1. Institut Charles Gerhardt Montpellier, Equipe MACS, UMR 5253 CNRS-ENSCM-UM2-UM1, 8 rue de l'Ecole, Normale, 34296 Montpellier Cedex 5, France
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  • Clarence Charnay

    Corresponding author
    1. Institut Charles Gerhardt Montpellier, Equipe Agrégats, Interfaces et, Matériaux pour l'Energie, UMR 5253 CNRS- Université Montpellier 2, C. C. 1502, Place Eugène Bataillon, 34095 Montpellier cedex 5, France
    • Institut Charles Gerhardt Montpellier, Equipe Agrégats, Interfaces et, Matériaux pour l'Energie, UMR 5253 CNRS- Université Montpellier 2, C. C. 1502, Place Eugène Bataillon, 34095 Montpellier cedex 5, France.
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Abstract

A simple synthetic route is developed to achieve gold functionalized radial mesoporous silica nanoparticles (Au-MsNP) synthesized by a one step procedure fully compatible with basic conditions required for the preparation of monodispersed nanospheres. In a second step, Au-MsNP particles have been coated with phospholipid bilayers in order to design an advanced biofunctional platform with the gold metallic nanoparticles previously grown into the pore channels and responsible for a plasmonic activity relevant for biosensing. The size of Au-MsNP is checked by dynamic light scattering while zeta potential measurements reflect their surface charge. The particle morphology is characterized by transmission and scanning electron microscopy and the Si/Au ratios are obtained from energy dispersive X-ray analysis. The textural properties of Au-MsNP, specific surface area and pore size, are determined from N2 adsorption. The supported bilayers are achieved from vesicles of different phospholipids incubated with Au-MsNP particles. The coating efficiency is investigated by zeta potential and cryo- transmission electron microscopy. The plasmonic activities of bare Au-MsNP particles and coated lipid bilayer Au-MsNP platform are evidenced for two model systems: direct adsorption of bovine serum albumin and molecular recognition events between avidin molecules and biotin receptors integrated in the supported lipid bilayer.

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