Communication

DNA–Protein Binding Force Chip

  1. Philip M. D. Severin1,2,
  2. Hermann E. Gaub1,2,*

Article first published online: 9 AUG 2012

DOI: 10.1002/smll.201201088

Issue

Small

Small

Volume 8, Issue 21, pages 3269–3273, November 5, 2012

Additional Information

How to Cite

Severin, P. M. D. and Gaub, H. E. (2012), DNA–Protein Binding Force Chip. Small, 8: 3269–3273. doi: 10.1002/smll.201201088

Author Information

  1. 1

    Lehrstuhl für Angewandte Physik and Center for Nanoscience (CeNS), Ludwig-Maximilians-Universität, Amalienstrasse 54, 80799 Munich, Germany

  2. 2

    Munich Center For Integrated Protein Science (CIPSM), Ludwig-Maximilians-Universität, Butenandtstrasse 5-13, 81377 Munich, Germany

*Lehrstuhl für Angewandte Physik and Center for Nanoscience (CeNS), Ludwig-Maximilians-Universität, Amalienstrasse 54, 80799 Munich, Germany.

Publication History

  1. Issue published online: 2 NOV 2012
  2. Article first published online: 9 AUG 2012
  3. Manuscript Received: 18 MAY 2012

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Keywords:

  • assays;
  • biophysics;
  • DNA-protein interactions;
  • microarrays;
  • molecular mechanics
Thumbnail image of graphical abstract

The binding force chip (BiFo-Chip) allows the quantification of DNA–protein interactions. It employs arrays of molecular force probes. Each molecular force probe functions comparable to a balance, which is influenced by the binding of a protein to one of the two DNA duplexes. The BiFo-chip is easy to operate, requires only a quantitative fluorescence microscope and fulfills all essential criteria for high throughput screening.

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