DNA–Protein Binding Force Chip

Authors

  • Philip M. D. Severin,

    1. Lehrstuhl für Angewandte Physik and Center for Nanoscience (CeNS), Ludwig-Maximilians-Universität, Amalienstrasse 54, 80799 Munich, Germany
    2. Munich Center For Integrated Protein Science (CIPSM), Ludwig-Maximilians-Universität, Butenandtstrasse 5-13, 81377 Munich, Germany
    Search for more papers by this author
  • Hermann E. Gaub

    Corresponding author
    1. Lehrstuhl für Angewandte Physik and Center for Nanoscience (CeNS), Ludwig-Maximilians-Universität, Amalienstrasse 54, 80799 Munich, Germany
    2. Munich Center For Integrated Protein Science (CIPSM), Ludwig-Maximilians-Universität, Butenandtstrasse 5-13, 81377 Munich, Germany
    • Lehrstuhl für Angewandte Physik and Center for Nanoscience (CeNS), Ludwig-Maximilians-Universität, Amalienstrasse 54, 80799 Munich, Germany.
    Search for more papers by this author

Abstract

original image

The binding force chip (BiFo-Chip) allows the quantification of DNA–protein interactions. It employs arrays of molecular force probes. Each molecular force probe functions comparable to a balance, which is influenced by the binding of a protein to one of the two DNA duplexes. The BiFo-chip is easy to operate, requires only a quantitative fluorescence microscope and fulfills all essential criteria for high throughput screening.

Ancillary