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High-Sensitivity Real-Time Analysis of Nanoparticle Toxicity in Green Fluorescent Protein-Expressing Zebrafish

Authors

  • Yu Pan,

    1. Helmholtz Institute for Biomedical Engineering, Biointerface Laboratory, RWTH Aachen University, Pauwelsstrasse 30, 52074 Aachen, Germany
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  • Annika Leifert,

    1. Institute of Inorganic Chemistry and JARA-Fundamentals of Future Information Technologies, RWTH Aachen University, 52056 Aachen, Germany
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  • Michael Graf,

    1. Helmholtz Institute for Biomedical Engineering, Biointerface Laboratory, RWTH Aachen University, Pauwelsstrasse 30, 52074 Aachen, Germany
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  • Frank Schiefer,

    1. Institute of Inorganic Chemistry and JARA-Fundamentals of Future Information Technologies, RWTH Aachen University, 52056 Aachen, Germany
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  • Sven Thoröe-Boveleth,

    1. RWTH Aachen University Hospital, Institute for Hygiene and Environmental Medicine, Pauwelsstrasse 30, 52074 Aachen, Germany
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  • Janine Broda,

    1. Institute of Inorganic Chemistry and JARA-Fundamentals of Future Information Technologies, RWTH Aachen University, 52056 Aachen, Germany
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  • Mary C. Halloran,

    1. Departments of Zoology and Neuroscience, University of Wisconsin, Madison, WI 53706, USA
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  • Henner Hollert,

    1. Department of Ecosystem Analysis, Institute for Environmental Research (Biology V), RWTH Aachen University, Worringerweg 1, 52074 Aachen, Germany
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  • Dominic Laaf,

    1. Helmholtz Institute for Biomedical Engineering, Biointerface Laboratory, RWTH Aachen University, Pauwelsstrasse 30, 52074 Aachen, Germany
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  • Ulrich Simon,

    1. Institute of Inorganic Chemistry and JARA-Fundamentals of Future Information Technologies, RWTH Aachen University, 52056 Aachen, Germany
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  • Willi Jahnen-Dechent

    Corresponding author
    1. Helmholtz Institute for Biomedical Engineering, Biointerface Laboratory, RWTH Aachen University, Pauwelsstrasse 30, 52074 Aachen, Germany
    • Helmholtz Institute for Biomedical Engineering, Biointerface Laboratory, RWTH Aachen University, Pauwelsstrasse 30, 52074 Aachen, Germany.
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Abstract

Gold nanoparticles (AuNP) show great potential for diagnostic and therapeutic application in humans. A great number of studies have tested the cytotoxicity of AuNP using cell culture. There is, however, an urgent need to test AuNP in vertebrate animal models that interrogate biodistribution and complex biological traits like organ development, whole body metabolism, and cognitive function. The sheer number of different compounds precludes the use of small rodent model for initial screening. The extended fish embryo test (FET) is used here to bridge the gap between cell culture and small animal models. A study on the toxicity of ultrasmall AuNP in wild type and transgenic zebrafish is presented. FET faithfully reproduce all important findings of a previous study in HeLa cells and add new important information on teratogenicity and hepatotoxicity that could not be gained from studying cultured cells.

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