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A Transparent Nanowire-Based Cell Impalement Device Suitable for Detailed Cell–Nanowire Interaction Studies

Authors

  • Florian Mumm,

    1. Department of Physics, Norwegian University of Science and Technology, Høgskoleringen 5, Trondheim, N-7491, Norway
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  • Kai M. Beckwith,

    1. Department of Physics, Norwegian University of Science and Technology, Høgskoleringen 5, Trondheim, N-7491, Norway
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  • Sara Bonde,

    1. Bio-Nanotechnology Laboratory, Department of Chemistry, University of Copenhagen, Universitetsparken 5, Copenhagen, DK-2100, Denmark
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  • Karen L. Martinez,

    1. Bio-Nanotechnology Laboratory, Department of Chemistry, University of Copenhagen, Universitetsparken 5, Copenhagen, DK-2100, Denmark
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  • Pawel Sikorski

    Corresponding author
    1. Department of Physics, Norwegian University of Science and Technology, Høgskoleringen 5, Trondheim, N-7491, Norway
    • Department of Physics, Norwegian University of Science and Technology, Høgskoleringen 5, Trondheim, N-7491, Norway.
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  • We would like to dedicate this paper to Florian Mumm, who unfortunately passed away just before the paper was submitted for publication. Florian played an essantial role in the research described here and he is greatly missed.

Abstract

A method to fabricate inexpensive and transparent nanowire impalement devices is invented based on CuO nanowire arrays grown by thermal oxidation. By employing a novel process the nanowires are transferred to a transparent, cell-compatible epoxy membrane. Cargo delivery and detailed cell-nanowire interaction studies are performed, revealing that the cell plasma membrane tightly wraps the nanowires, while cell membrane penetration is not observed. The presented device offers an efficient investigation platform for further optimization, leading towards a simple and versatile impalement delivery system.

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