Short Multiwall Carbon Nanotubes Promote Neuronal Differentiation of PC12 Cells via Up-Regulation of the Neurotrophin Signaling Pathway

Authors

  • Li Meng,

    1. CAS Key Laboratory for Biomedical Effects of Nanomaterials & Nanosafety, National Center for Nanoscience and Technology of China, Beijing 100190, China
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  • Rui Chen,

    1. CAS Key Laboratory for Biomedical Effects of Nanomaterials & Nanosafety, National Center for Nanoscience and Technology of China, Beijing 100190, China
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  • Aihua Jiang,

    1. CAS Key Laboratory for Biomedical Effects of Nanomaterials & Nanosafety, National Center for Nanoscience and Technology of China, Beijing 100190, China
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  • Liming Wang,

    1. CAS Key Laboratory for Biomedical Effects of Nanomaterials & Nanosafety, Institute of High Energy Physics, Chinese Academy of Sciences (CAS), Beijing 100049, China
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  • Peng Wang,

    1. CAS Key Laboratory for Biomedical Effects of Nanomaterials & Nanosafety, National Center for Nanoscience and Technology of China, Beijing 100190, China
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  • Chen-zhong Li,

    1. Nanobioengineering/Bioelectronics Lab, Department of Biomedical Engineering, Florida International University, Miami, USA
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  • Ru Bai,

    1. CAS Key Laboratory for Biomedical Effects of Nanomaterials & Nanosafety, National Center for Nanoscience and Technology of China, Beijing 100190, China
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  • Yuliang Zhao,

    1. CAS Key Laboratory for Biomedical Effects of Nanomaterials & Nanosafety, National Center for Nanoscience and Technology of China, Beijing 100190, China
    2. CAS Key Laboratory for Biomedical Effects of Nanomaterials & Nanosafety, Institute of High Energy Physics, Chinese Academy of Sciences (CAS), Beijing 100049, China
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  • Herman Autrup,

    1. Department of Public Health, Aarhus University, Aarhus, Denmark
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  • Chunying Chen

    Corresponding author
    1. CAS Key Laboratory for Biomedical Effects of Nanomaterials & Nanosafety, National Center for Nanoscience and Technology of China, Beijing 100190, China
    • CAS Key Laboratory for Biomedical Effects of Nanomaterials & Nanosafety, National Center for Nanoscience and Technology of China, Beijing 100190, China.
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Abstract

Numerous unique properties of carbon nanotubes make them attractive for applications in neurobiology such as drug delivery, tissue regeneration, and as scaffolds for neuronal growth. In this study, the critical roles of the length of multiwall carbon nanotubes (MWCNTs) on a neuronal-like model cell line PC12 cells are investiaged. Incubation of PC12 cells with carboxylated MWCNTs did not significantly affect cellular morphology and viability at lower concentrations. Short MWCNTs show higher cellular uptake and more obvious removal compared to longer ones, which can result in higher ability to promote PC12 cell differentiation. Pre-incubation of short MWCNTs can up-regulate the expression of neurotrophin signaling pathway-associated TrkA/p75 receptors and Pincher/Gap43/TH proteins, which might be the underlying mechanism for the improved differentiation in PC12 cells. The current results provide insight for future applications of MWCNTs in neuron drug delivery and neurodegenerative disease treatment.

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