Nanoengineered Colloidal Probes for Raman-based Detection of Biomolecules inside Living Cells

Authors

  • Alexey Yashchenok,

    1. Max Planck Institute of Colloids and Interfaces, Department of Interfaces, 14424 Potsdam, Germany
    2. Saratov State University, Department of Nano- and Biomedical Technologies, 410012 Saratov, Russia
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  • Admir Masic,

    Corresponding author
    1. Max-Planck Institute of Colloids and Interfaces, Department of Biomaterials, 14424 Potsdam, Germany
    • Max-Planck Institute of Colloids and Interfaces, Department of Biomaterials, 14424 Potsdam, Germany.
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  • Dmitry Gorin,

    1. Max Planck Institute of Colloids and Interfaces, Department of Interfaces, 14424 Potsdam, Germany
    2. Saratov State University, Department of Nano- and Biomedical Technologies, 410012 Saratov, Russia
    3. Saratov State University, Institute of Nanostructures and Biosystems, 410012 Saratov, Russia
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  • Bong Sup Shim,

    1. University of Michigan, Department of Chemical Engineering, Department of Materials Science, Department of Biomedical Engineering, Biointerface Institute, Ann Arbor, MI 48109, USA
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  • Nicholas A. Kotov,

    1. University of Michigan, Department of Chemical Engineering, Department of Materials Science, Department of Biomedical Engineering, Biointerface Institute, Ann Arbor, MI 48109, USA
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  • Peter Fratzl,

    1. Max-Planck Institute of Colloids and Interfaces, Department of Biomaterials, 14424 Potsdam, Germany
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  • Helmuth Möhwald,

    1. Max Planck Institute of Colloids and Interfaces, Department of Interfaces, 14424 Potsdam, Germany
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  • Andre Skirtach

    Corresponding author
    1. Max Planck Institute of Colloids and Interfaces, Department of Interfaces, 14424 Potsdam, Germany
    2. Department of Molecular Biotechnology, Ghent University, 9000 Ghent, and Nano-Bio, (NB)-Photonics, Ghent University, 9000 Ghent, Belgium
    • Max Planck Institute of Colloids and Interfaces, Department of Interfaces, 14424 Potsdam, Germany
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Abstract

original image

Gold nanoparticle aggregate carbon nanotube functionalized colloidal particles serve as an efficient platform for probing the intracellular environment. The probes provide the means of effective localization of signal and detection of molecular fingerprints of biomolecules in living cells. The approach demonstrated in this work opens significant opportunities in molecular imaging as well as intracellular sensing and trafficking.

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