Get access
Advertisement

Quantifying and Imaging Engineered Nanomaterials In Vivo: Challenges and Techniques

Authors

  • Xiao He,

    1. CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety and CAS Key Laboratory of Nuclear Analytical Techniques, Institute of High Energy Physics, Chinese Academy of Sciences (CAS), Beijing 100049, China
    Search for more papers by this author
  • Yuhui Ma,

    1. CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety and CAS Key Laboratory of Nuclear Analytical Techniques, Institute of High Energy Physics, Chinese Academy of Sciences (CAS), Beijing 100049, China
    Search for more papers by this author
  • Meng Li,

    1. CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety and CAS Key Laboratory of Nuclear Analytical Techniques, Institute of High Energy Physics, Chinese Academy of Sciences (CAS), Beijing 100049, China
    Search for more papers by this author
  • Peng Zhang,

    1. CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety and CAS Key Laboratory of Nuclear Analytical Techniques, Institute of High Energy Physics, Chinese Academy of Sciences (CAS), Beijing 100049, China
    Search for more papers by this author
  • Yuanyuan Li,

    1. CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety and CAS Key Laboratory of Nuclear Analytical Techniques, Institute of High Energy Physics, Chinese Academy of Sciences (CAS), Beijing 100049, China
    Search for more papers by this author
  • Zhiyong Zhang

    Corresponding author
    1. CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety and CAS Key Laboratory of Nuclear Analytical Techniques, Institute of High Energy Physics, Chinese Academy of Sciences (CAS), Beijing 100049, China
    • CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety and CAS Key Laboratory of Nuclear Analytical Techniques, Institute of High Energy Physics, Chinese Academy of Sciences (CAS), Beijing 100049, China.
    Search for more papers by this author

Abstract

Quantifying and imaging the engineered nanomaterials (ENMs) in vivo can provide information on the bio-distribution and fate of ENMs in living systems. A necessary amount of in vivo quantitative data is indispensable to verify the extrapolation from in vitro tests, to modify the predictive models of ENM exposure, and to underpin the risk management strategy for ENMs. However, it remains a challenge to quantitatively assess the bio-distribution of ENMs under realistic exposure, their long-term deposition (especially in non-targeted tissues), their passage across the natural barriers, and the impacts of nano–bio interactions on their in vivo behaviors. Some commonly used techniques for in vivo ENM quantification, such as electron microscopy, fluorescence-based detection, atomic spectroscopy, radiotracing, and techniques basing on synchrotron radiation are reviewed, and their technical characteristics, the state of the art, limitations, and future prospects are addressed.

Get access to the full text of this article

Ancillary