Adsorption of Multimeric T Cell Antigens on Carbon Nanotubes: Effect on Protein Structure and Antigen-Specific T Cell Stimulation

Authors

  • Tarek R. Fadel,

    1. Departments of Chemical and Biomedical Engineering, Yale University, P.O. Box 208284, New Haven, Connecticut, USA, Fax: (+1) 203 432-0030
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  • Nan Li,

    1. Departments of Chemical and Biomedical Engineering, Yale University, P.O. Box 208284, New Haven, Connecticut, USA, Fax: (+1) 203 432-0030
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  • Smith Shah,

    1. Departments of Chemical and Biomedical Engineering, Yale University, P.O. Box 208284, New Haven, Connecticut, USA, Fax: (+1) 203 432-0030
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  • Michael Look,

    1. Departments of Chemical and Biomedical Engineering, Yale University, P.O. Box 208284, New Haven, Connecticut, USA, Fax: (+1) 203 432-0030
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  • Lisa D. Pfefferle,

    1. Departments of Chemical and Biomedical Engineering, Yale University, P.O. Box 208284, New Haven, Connecticut, USA, Fax: (+1) 203 432-0030
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  • Gary L. Haller,

    1. Departments of Chemical and Biomedical Engineering, Yale University, P.O. Box 208284, New Haven, Connecticut, USA, Fax: (+1) 203 432-0030
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  • Sune Justesen,

    1. Department of Immunobiology and Microbiology, The Panum Institute Blegdamsvej 3b, 2200 N, Copenhagen, Denmark
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  • Corey J. Wilson,

    Corresponding author
    1. Departments of Chemical and Biomedical Engineering, Yale University, P.O. Box 208284, New Haven, Connecticut, USA, Fax: (+1) 203 432-0030
    • Departments of Chemical and Biomedical Engineering, Yale University, P.O. Box 208284, New Haven, Connecticut, USA, Fax: (+1) 203 432-0030.
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  • Tarek M. Fahmy

    Corresponding author
    1. Departments of Chemical and Biomedical Engineering, Yale University, P.O. Box 208284, New Haven, Connecticut, USA, Fax: (+1) 203 432-0030
    • Departments of Chemical and Biomedical Engineering, Yale University, P.O. Box 208284, New Haven, Connecticut, USA, Fax: (+1) 203 432-0030.
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Abstract

original image

Antigen-specific activation of cytotoxic T cells can be enhanced up to three-fold more than soluble controls when using functionalized bundled carbon nanotube substrates (bCNTs). To overcome the denaturing effects of direct adsorption on bCNTs, a simple but robust method is demonstrated to stabilize the T cell stimulus on carbon nanotube substrates through non-covalent attachment of the linker neutravidin.

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