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Quantum Dot-Based Thermal Spectroscopy and Imaging of Optically Trapped Microspheres and Single Cells

Authors

  • Patricia Haro-González,

    1. Laboratorio di Chimica dello Stato Solido, DB, Università di Verona and INSTM, UdR Verona, Ca' Vignal, Strada Le Grazie 15, I-37134 Verona, Italy
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  • William T. Ramsay,

    1. Scottish Universities Physics Alliance (SUPA), Institute of Biological Chemistry, Biophysics and Bioengineering, School of Engineering & Physical Sciences, Heriot Watt University, Edinburgh EH14 4AS, Midlothian, Scotland
    Current affiliation:
    1. These authors contributed equally to this work.
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  • Laura Martinez Maestro,

    1. Fluorescence Imaging Group, Departamento de Física de Materiales, C-IV, Universidad Autónoma de Madrid C/Francisco Tomás y Valiente 7, Madrid, 28049, Spain
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  • Blanca del Rosal,

    1. Fluorescence Imaging Group, Departamento de Física de Materiales, C-IV, Universidad Autónoma de Madrid C/Francisco Tomás y Valiente 7, Madrid, 28049, Spain
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  • Karla Santacruz-Gomez,

    1. Centro de Investigación en Materiales Avanzados, CIMAV, Departmento de Fisica, Universidad de Sonora, A.P 1626 Hermosillo, Sonora, México
    2. Departamento de Física, Universidad de Sonora, Blvd. Luis Encinas y Rosales s/n, C.P. 83000, Hermosillo, Sonora, México
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  • Maria del Carmen Iglesias-de la Cruz,

    1. Fluorescence Imaging Group, Departamento de Física de Materiales, C-IV, Universidad Autónoma de Madrid C/Francisco Tomás y Valiente 7, Madrid, 28049, Spain
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  • Francisco Sanz-Rodríguez,

    1. Fluorescence Imaging Group, Departamento de Física de Materiales, C-IV, Universidad Autónoma de Madrid C/Francisco Tomás y Valiente 7, Madrid, 28049, Spain
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  • Jing Yuang Chooi,

    1. Fluorescence Imaging Group, Departamento de Física de Materiales, C-IV, Universidad Autónoma de Madrid C/Francisco Tomás y Valiente 7, Madrid, 28049, Spain
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  • Paloma Rodriguez Sevilla,

    1. Fluorescence Imaging Group, Departamento de Física de Materiales, C-IV, Universidad Autónoma de Madrid C/Francisco Tomás y Valiente 7, Madrid, 28049, Spain
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  • Marco Bettinelli,

    1. Laboratorio di Chimica dello Stato Solido, DB, Università di Verona and INSTM, UdR Verona, Ca' Vignal, Strada Le Grazie 15, I-37134 Verona, Italy
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  • Debaditya Choudhury,

    1. Scottish Universities Physics Alliance (SUPA), Institute of Biological Chemistry, Biophysics and Bioengineering, School of Engineering & Physical Sciences, Heriot Watt University, Edinburgh EH14 4AS, Midlothian, Scotland
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  • Ajoy K. Kar,

    1. Scottish Universities Physics Alliance (SUPA), Institute of Photonic and Quantum Sciences, School of Engineering & Physical Sciences, Heriot Watt University, Edinburgh EH14 4AS, Midlothian, Scotland
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  • José García Solé,

    1. Fluorescence Imaging Group, Departamento de Física de Materiales, C-IV, Universidad Autónoma de Madrid C/Francisco Tomás y Valiente 7, Madrid, 28049, Spain
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  • Daniel Jaque,

    1. Fluorescence Imaging Group, Departamento de Física de Materiales, C-IV, Universidad Autónoma de Madrid C/Francisco Tomás y Valiente 7, Madrid, 28049, Spain
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  • Lynn Paterson

    Corresponding author
    1. Scottish Universities Physics Alliance (SUPA), Institute of Biological Chemistry, Biophysics and Bioengineering, School of Engineering & Physical Sciences, Heriot Watt University, Edinburgh EH14 4AS, Midlothian, Scotland
    • Scottish Universities Physics Alliance (SUPA), Institute of Biological Chemistry, Biophysics and Bioengineering, School of Engineering & Physical Sciences, Heriot Watt University, Edinburgh EH14 4AS, Midlothian, Scotland.
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Abstract

Laser-induced thermal effects in optically trapped microspheres and single cells are investigated by quantum dot luminescence thermometry. Thermal spectroscopy has revealed a non-localized temperature distribution around the trap that extends over tens of micrometers, in agreement with previous theoretical models besides identifying water absorption as the most important heating source. The experimental results of thermal loading at a variety of wavelengths reveal that an optimum trapping wavelength exists for biological applications close to 820 nm. This is corroborated by a simultaneous analysis of the spectral dependence of cellular heating and damage in human lymphocytes during optical trapping. This quantum dot luminescence thermometry demonstrates that optical trapping with 820 nm laser radiation produces minimum intracellular heating, well below the cytotoxic level (43 °C), thus, avoiding cell damage.

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