Involvement of Lysosomal Exocytosis in the Excretion of Mesoporous Silica Nanoparticles and Enhancement of the Drug Delivery Effect by Exocytosis Inhibition

Authors

  • Rolando E. Yanes,

    1. Department of Microbiology, Immunology and Molecular Genetics, California NanoSystems Institute, Jonsson Comprehensive Cancer Center, University of California, Los Angeles, 405 Hilgard Avenue, Los Angeles, CA 90095, USA, Tel: (+1)-310-206-7318, Fax: (+1)310-206-5231
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  • Derrick Tarn,

    1. Department of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, CA 90095, USA
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  • Angela A. Hwang,

    1. Department of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, CA 90095, USA
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  • Daniel P. Ferris,

    1. Department of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, CA 90095, USA
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  • Sean P. Sherman,

    1. Molecular Biology Institute, University of California, Los Angeles, Los Angeles, CA 90095, USA
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  • Courtney R. Thomas,

    1. Department of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, CA 90095, USA
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  • Jie Lu,

    1. Department of Microbiology, Immunology and Molecular Genetics, California NanoSystems Institute, Jonsson Comprehensive Cancer Center, University of California, Los Angeles, 405 Hilgard Avenue, Los Angeles, CA 90095, USA, Tel: (+1)-310-206-7318, Fax: (+1)310-206-5231
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  • April D. Pyle,

    1. Department of Microbiology, Immunology and Molecular Genetics, California NanoSystems Institute, Jonsson Comprehensive Cancer Center, University of California, Los Angeles, 405 Hilgard Avenue, Los Angeles, CA 90095, USA, Tel: (+1)-310-206-7318, Fax: (+1)310-206-5231
    2. Molecular Biology Institute, University of California, Los Angeles, Los Angeles, CA 90095, USA
    3. Eli and Edythe Broad Center of Regenerative, Medicine and Stem Cell Research, University of California, Los Angeles, Los Angeles, CA 90095, USA
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  • Jeffrey I. Zink,

    1. Department of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, CA 90095, USA
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  • Fuyuhiko Tamanoi

    Corresponding author
    1. Department of Microbiology, Immunology and Molecular Genetics, California NanoSystems Institute, Jonsson Comprehensive Cancer Center, University of California, Los Angeles, 405 Hilgard Avenue, Los Angeles, CA 90095, USA, Tel: (+1)-310-206-7318, Fax: (+1)310-206-5231
    • Department of Microbiology, Immunology and Molecular Genetics, California NanoSystems Institute, Jonsson Comprehensive Cancer Center, University of California, Los Angeles, 405 Hilgard Avenue, Los Angeles, CA 90095, USA, Tel: (+1)-310-206-7318, Fax: (+1)310-206-5231.
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Abstract

original image

The exocytosis of phosphonate modified mesoporous silica nanoparticles (P-MSNs) is demonstrated and lysosomal exocytosis is identified as the mechanism responsible for this event. Regulation of P-MSN exocytosis can be achieved by inhibiting or accelerating lysosomal exocytosis. Slowing down P-MSN exocytosis enhances the drug delivery effect of CPT-loaded P-MSNs by improving cell killing.

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