Microglia Response and In Vivo Therapeutic Potential of Methylprednisolone-Loaded Dendrimer Nanoparticles in Spinal Cord Injury

Authors

  • Susana R. Cerqueira,

    Corresponding author
    1. 3B's Research Group–Biomaterials, Biodegradables and Biomimetics, University of Minho, AvePark, Zona Industrial da Gandra, S. Cláudio do Barco, 4806-909 Caldas das Taipas, Guimarães, Portugal
    2. ICVS/3B's - PT Government Associate Lab, University of Minho, 4710-057 Braga, Portugal
    3. Life and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho, 4710-057 Braga, Portugal
    • 3B's Research Group–Biomaterials, Biodegradables and Biomimetics, University of Minho, AvePark, Zona Industrial da Gandra, S. Cláudio do Barco, 4806-909 Caldas das Taipas, Guimarães, Portugal
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  • Joaquim M. Oliveira,

    1. 3B's Research Group–Biomaterials, Biodegradables and Biomimetics, University of Minho, AvePark, Zona Industrial da Gandra, S. Cláudio do Barco, 4806-909 Caldas das Taipas, Guimarães, Portugal
    2. ICVS/3B's - PT Government Associate Lab, University of Minho, 4710-057 Braga, Portugal
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  • Nuno A. Silva,

    1. 3B's Research Group–Biomaterials, Biodegradables and Biomimetics, University of Minho, AvePark, Zona Industrial da Gandra, S. Cláudio do Barco, 4806-909 Caldas das Taipas, Guimarães, Portugal
    2. ICVS/3B's - PT Government Associate Lab, University of Minho, 4710-057 Braga, Portugal
    3. Life and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho, 4710-057 Braga, Portugal
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  • Hugo Leite-Almeida,

    1. ICVS/3B's - PT Government Associate Lab, University of Minho, 4710-057 Braga, Portugal
    2. Life and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho, 4710-057 Braga, Portugal
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  • Silvina Ribeiro-Samy,

    1. ICVS/3B's - PT Government Associate Lab, University of Minho, 4710-057 Braga, Portugal
    2. Life and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho, 4710-057 Braga, Portugal
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  • Armando Almeida,

    1. ICVS/3B's - PT Government Associate Lab, University of Minho, 4710-057 Braga, Portugal
    2. Life and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho, 4710-057 Braga, Portugal
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  • João F. Mano,

    1. 3B's Research Group–Biomaterials, Biodegradables and Biomimetics, University of Minho, AvePark, Zona Industrial da Gandra, S. Cláudio do Barco, 4806-909 Caldas das Taipas, Guimarães, Portugal
    2. ICVS/3B's - PT Government Associate Lab, University of Minho, 4710-057 Braga, Portugal
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  • Nuno Sousa,

    1. ICVS/3B's - PT Government Associate Lab, University of Minho, 4710-057 Braga, Portugal
    2. Life and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho, 4710-057 Braga, Portugal
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  • António J. Salgado,

    Corresponding author
    1. ICVS/3B's - PT Government Associate Lab, University of Minho, 4710-057 Braga, Portugal
    2. Life and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho, 4710-057 Braga, Portugal
    • Life and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho, 4710-057 Braga, Portugal.
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  • Rui L. Reis

    1. 3B's Research Group–Biomaterials, Biodegradables and Biomimetics, University of Minho, AvePark, Zona Industrial da Gandra, S. Cláudio do Barco, 4806-909 Caldas das Taipas, Guimarães, Portugal
    2. ICVS/3B's - PT Government Associate Lab, University of Minho, 4710-057 Braga, Portugal
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Errata

This article is corrected by:

  1. Errata: Microglia Response and In Vivo Therapeutic Potential of Methylprednisolone-Loaded Dendrimer Nanoparticles in Spinal Cord Injury Volume 12, Issue 8, 972, Article first published online: 18 January 2016

Abstract

The control and manipulation of cells that trigger secondary mechanisms following spinal cord injury (SCI) is one of the first opportunities to minimize its highly detrimental outcomes. Herein, the ability of surface-engineered carboxymethylchitosan/polyamidoamine (CMCht/PAMAM) dendrimer nanoparticles to intracellularly deliver methylprednisolone (MP) to glial cells, allowing a controlled and sustained release of this corticosteroid in the injury site, is investigated. The negatively charged MP-loaded CMCht/PAMAM dendrimer nanoparticles with sizes of 109 nm enable a MP sustained release, which is detected for a period of 14 days by HPLC. In vitro studies in glial primary cultures show that incubation with 200 μg mL−1 nanoparticles do not affect the cells' viability or proliferation, while allowing the entire population to internalize the nanoparticles. At higher concentrations, microglial cell viability is proven to be affected in response to the MP amount released. Following lateral hemisection lesions in rats, nanoparticle uptake by the spinal tissue is observed 3 h after administration. Moreover, significant differences in the locomotor output between the controls and the MP-loaded nanoparticle-treated animals one month after the lesion are observed. Therefore, MP-loaded CMCht/PAMAM dendrimer nanoparticles may prove to be useful in the reduction of the secondary injury following SCI.

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