Get access

Photosensitizer–Gold Nanorod Composite for Targeted Multimodal Therapy

Authors

  • Jian Wang,

    1. Center for Research at the Bio/Nano Interface, Department of Chemistry and Shands Cancer Center, University of Florida Genetics Institute, University of Florida, Gainesville, FL 32611-7200, USA
    2. Ministry of Education Key Laboratory on Luminescence and Real-Time Analysis, College of Pharmaceutical Sciences, Southwest University, Chongqing, 400715, China
    Search for more papers by this author
  • Mingxu You,

    1. Center for Research at the Bio/Nano Interface, Department of Chemistry and Shands Cancer Center, University of Florida Genetics Institute, University of Florida, Gainesville, FL 32611-7200, USA
    Search for more papers by this author
  • Guizhi Zhu,

    1. Center for Research at the Bio/Nano Interface, Department of Chemistry and Shands Cancer Center, University of Florida Genetics Institute, University of Florida, Gainesville, FL 32611-7200, USA
    Search for more papers by this author
  • Mohammed Ibrahim Shukoor,

    1. Center for Research at the Bio/Nano Interface, Department of Chemistry and Shands Cancer Center, University of Florida Genetics Institute, University of Florida, Gainesville, FL 32611-7200, USA
    Search for more papers by this author
  • Zhuo Chen,

    1. Molecular Sciences and Biomedicine Laboratory, State Key Laboratory for Chemo/Biosensing and Chemometrics, College of Biology and College of Chemistry and Chemical Engineering, Collaborative Innovation Center for Chemistry and Molecular Medicine, Hunan University, Changsha 410082, China
    Search for more papers by this author
  • Zilong Zhao,

    1. Molecular Sciences and Biomedicine Laboratory, State Key Laboratory for Chemo/Biosensing and Chemometrics, College of Biology and College of Chemistry and Chemical Engineering, Collaborative Innovation Center for Chemistry and Molecular Medicine, Hunan University, Changsha 410082, China
    Search for more papers by this author
  • Meghan B. Altman,

    1. Center for Research at the Bio/Nano Interface, Department of Chemistry and Shands Cancer Center, University of Florida Genetics Institute, University of Florida, Gainesville, FL 32611-7200, USA
    Search for more papers by this author
  • Quan Yuan,

    1. Molecular Sciences and Biomedicine Laboratory, State Key Laboratory for Chemo/Biosensing and Chemometrics, College of Biology and College of Chemistry and Chemical Engineering, Collaborative Innovation Center for Chemistry and Molecular Medicine, Hunan University, Changsha 410082, China
    Search for more papers by this author
  • Zhi Zhu,

    1. Molecular Sciences and Biomedicine Laboratory, State Key Laboratory for Chemo/Biosensing and Chemometrics, College of Biology and College of Chemistry and Chemical Engineering, Collaborative Innovation Center for Chemistry and Molecular Medicine, Hunan University, Changsha 410082, China
    Search for more papers by this author
  • Yan Chen,

    1. Molecular Sciences and Biomedicine Laboratory, State Key Laboratory for Chemo/Biosensing and Chemometrics, College of Biology and College of Chemistry and Chemical Engineering, Collaborative Innovation Center for Chemistry and Molecular Medicine, Hunan University, Changsha 410082, China
    Search for more papers by this author
  • Cheng Zhi Huang,

    Corresponding author
    1. Ministry of Education Key Laboratory on Luminescence and Real-Time Analysis, College of Pharmaceutical Sciences, Southwest University, Chongqing, 400715, China
    • Cheng Zhi Huang, Ministry of Education Key Laboratory on Luminescence and Real-Time Analysis, College of Pharmaceutical Sciences, Southwest University, Chongqing, 400715, China.

      Weihong Tan, Center for Research at the Bio/Nano Interface, Department of Chemistry and Shands Cancer Center, University of Florida Genetics Institute, University of Florida, Gainesville, FL 32611-7200, USA

    Search for more papers by this author
  • Weihong Tan

    Corresponding author
    1. Center for Research at the Bio/Nano Interface, Department of Chemistry and Shands Cancer Center, University of Florida Genetics Institute, University of Florida, Gainesville, FL 32611-7200, USA
    2. Molecular Sciences and Biomedicine Laboratory, State Key Laboratory for Chemo/Biosensing and Chemometrics, College of Biology and College of Chemistry and Chemical Engineering, Collaborative Innovation Center for Chemistry and Molecular Medicine, Hunan University, Changsha 410082, China
    • Cheng Zhi Huang, Ministry of Education Key Laboratory on Luminescence and Real-Time Analysis, College of Pharmaceutical Sciences, Southwest University, Chongqing, 400715, China.

      Weihong Tan, Center for Research at the Bio/Nano Interface, Department of Chemistry and Shands Cancer Center, University of Florida Genetics Institute, University of Florida, Gainesville, FL 32611-7200, USA

    Search for more papers by this author

Abstract

In this work, a DNA inter-strand replacement strategy for therapeutic activity is successfully designed for multimodal therapy. In this multimodal therapy, chlorin e6 (Ce6) photosensitizer molecules are used for photodynamic therapy (PDT), while aptamer-AuNRs, are used for selective binding to target cancer cells and for photothermal therapy (PTT) with near infrared laser irradiation. Aptamer Sgc8, which specifically targets leukemia T cells, is conjugated to an AuNR by a thiol-Au covalent bond and then hybridized with a Ce6-labeled photosensitizer/reporter to form a DNA double helix. When target cancer cells are absent, Ce6 is quenched and shows no PDT effect. However, when target cancer cells are present, the aptamer changes structure to release Ce6 to produce singlet oxygen for PDT upon light irradiation. Importantly, by combining photosensitizer and photothermal agents, PTT/PDT dual therapy supplies a more effective therapeutic outcome than either therapeutic modality alone.

Get access to the full text of this article

Ancillary