SEARCH

SEARCH BY CITATION

Keywords:

  • atomic force microscopy;
  • intranuclear injection;
  • microfluidics;
  • single cells;
  • transfection
Thumbnail image of graphical abstract

Surpassing the physical barriers of the cytoplasmic and nuclear membranes to deliver biomolecules directly into cell nuclei offers opportunities to investigate dynamic processes in living cells. The potential of atomic force microscopy coupled to microfluidics (FluidFM) for volume-controlled intranuclear delivery is demonstrated, whereby minimally invasive microchanneled probes are remotely driven with high spatiotemporal resolution.