Modulation of Lumen Formation by Microgeometrical Bioactive Cues and Migration Mode of Actin Machinery

Authors

  • Yifeng Lei,

    Corresponding author
    1. Université Bordeaux 1-CNRS, UMR5248, Institut Européen de Chimie et Biologie, 2, rue Robert Escarpit, F-33607 Pessac, France
    • Université Bordeaux 1-CNRS, UMR5248, Institut Européen de Chimie et Biologie, 2, rue Robert Escarpit, F-33607 Pessac, France.
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  • Omar F. Zouani,

    Corresponding author
    1. Université Bordeaux 1-CNRS, UMR5248, Institut Européen de Chimie et Biologie, 2, rue Robert Escarpit, F-33607 Pessac, France
    Current affiliation:
    1. OFZ conceived and supervised this work and is the principal investigator.
    • Université Bordeaux 1-CNRS, UMR5248, Institut Européen de Chimie et Biologie, 2, rue Robert Escarpit, F-33607 Pessac, France.
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  • Lila Rami,

    1. Université Bordeaux 2, Inserm U1026, Bioingénierie Tissulaire, 146, rue Léo Saignat, F-33076 Bordeaux, France
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  • Christel Chanseau,

    1. Université Bordeaux 2, Inserm U1026, Bioingénierie Tissulaire, 146, rue Léo Saignat, F-33076 Bordeaux, France
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  • Marie-Christine Durrieu

    1. Université Bordeaux 1-CNRS, UMR5248, Institut Européen de Chimie et Biologie, 2, rue Robert Escarpit, F-33607 Pessac, France
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Abstract

How endothelial cells (ECs) express the particular filopodial or lamellipodial form of the actin machinery is critical to understanding EC functions such as angiogenesis and sprouting. It is not known how these mechanisms coordinately promote lumen formation of ECs. Here, adhesion molecules (RGD peptides) and inductor molecules (BMP-2 mimetic peptides) are micropatterned onto polymer surfaces by a photolithographic technique to induce filopodial and lamellipodial migration modes. Firstly, the effects of peptide microgeometrical distribution on EC adhesion, orientation and morphogenesis are evaluated. Large micropatterns (100 μm) promote EC orientation without lumen formation, whereas small micropatterns (10–50 μm) elicit a collective cell organization and induce EC lumen formation, in the case of RGD peptides. Secondly, the correlation between EC actin machinery expression and EC self-assembly into lumen formation is addressed. Only the filopodial migration mode (mimicked by RGD) but not lamellipodial migration mode (mimicked by BMP-2) promotes EC lumen formation. This work gives a new concept for the design of biomaterials for tissue engineering and may provide new insight for angiogenesis inhibition on tumors.

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