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Three Dimensional, Sheathless, and High-Throughput Microparticle Inertial Focusing Through Geometry-Induced Secondary Flows

Authors

  • Aram J. Chung,

    1. Department of Bioengineering, California NanoSystems Institute, University of California, Los Angeles, CA 90095, USA, Phone: (310) 983-3235, Fax: (310) 794-5956
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  • Daniel R. Gossett,

    1. Department of Bioengineering, California NanoSystems Institute, University of California, Los Angeles, CA 90095, USA, Phone: (310) 983-3235, Fax: (310) 794-5956
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  • Dino Di Carlo

    Corresponding author
    1. Department of Bioengineering, California NanoSystems Institute, University of California, Los Angeles, CA 90095, USA, Phone: (310) 983-3235, Fax: (310) 794-5956
    • Department of Bioengineering, California NanoSystems Institute, University of California, Los Angeles, CA 90095, USA, Phone: (310) 983-3235, Fax: (310) 794-5956.
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Abstract

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A novel inertial focusing platform creates a single-stream microparticle train in a single-focal plane without sheath fluids and external forces, all in a high-throughput manner. The proposed design consists of a low-aspect-ratio straight channel interspersed with a series of constrictions in height arranged orthogonally, making use of inertial focusing and geometry-induced secondary flows. Focusing efficiency as high as 99.77% is demonstrated with throughput as high as 36 000 particles s−1 for a variety of different sized particles and cells.

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