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A Molecular Beacon-Based Signal-Off Surface-Enhanced Raman Scattering Strategy for Highly Sensitive, Reproducible, and Multiplexed DNA Detection

Authors

  • Xinpan Wei,

    1. Institute of Functional Nano & Soft Materials (FUNSOM) and Jiangsu Key Laboratory for Carbon-Based Functional Materials & Devices, Soochow University, Suzhou 215123, PR China
    Current affiliation:
    1. These authors contributed equally to this work.
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  • Shao Su,

    1. Institute of Functional Nano & Soft Materials (FUNSOM) and Jiangsu Key Laboratory for Carbon-Based Functional Materials & Devices, Soochow University, Suzhou 215123, PR China
    2. Laboratory of Physical Biology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, PR China
    Current affiliation:
    1. These authors contributed equally to this work.
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  • Yuanyuan Guo,

    1. Institute of Functional Nano & Soft Materials (FUNSOM) and Jiangsu Key Laboratory for Carbon-Based Functional Materials & Devices, Soochow University, Suzhou 215123, PR China
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  • Xiangxu Jiang,

    1. Institute of Functional Nano & Soft Materials (FUNSOM) and Jiangsu Key Laboratory for Carbon-Based Functional Materials & Devices, Soochow University, Suzhou 215123, PR China
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  • Yiling Zhong,

    1. Institute of Functional Nano & Soft Materials (FUNSOM) and Jiangsu Key Laboratory for Carbon-Based Functional Materials & Devices, Soochow University, Suzhou 215123, PR China
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  • Yuanyuan Su,

    1. Institute of Functional Nano & Soft Materials (FUNSOM) and Jiangsu Key Laboratory for Carbon-Based Functional Materials & Devices, Soochow University, Suzhou 215123, PR China
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  • Chunhai Fan,

    1. Laboratory of Physical Biology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, PR China
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  • Shuit-Tong Lee,

    1. Institute of Functional Nano & Soft Materials (FUNSOM) and Jiangsu Key Laboratory for Carbon-Based Functional Materials & Devices, Soochow University, Suzhou 215123, PR China
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  • Yao He

    Corresponding author
    1. Institute of Functional Nano & Soft Materials (FUNSOM) and Jiangsu Key Laboratory for Carbon-Based Functional Materials & Devices, Soochow University, Suzhou 215123, PR China
    • Institute of Functional Nano & Soft Materials (FUNSOM) and Jiangsu Key Laboratory for Carbon-Based Functional Materials & Devices, Soochow University, Suzhou 215123, PR China.
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Abstract

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Stem-loop oligonucleotides tagged with organic dyes are employed as capture and reporter probes in a molecular beacon-based signal-off surface- enhanced Raman scattering (SERS) strategy. DNA strands are immobilized on a gold nanoparticle-decorated silicon nanowire array (AuNPs@SiNWAr). DNA with a concentration down to ≈10 fM is detected under irradiation from a low-power laser, which is better than or comparable to that detected by signal-on SERS methods. The signal-off strategy allows identification of single-base mismatches and detection of multiplexed DNA.

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