Bifunctional Graphene/γ-Fe2O3 Hybrid Aerogels with Double Nanocrystalline Networks for Enzyme Immobilization

Authors

  • Liang Chen,

    1. School of Materials Science & Engineering, Beijing Institute of Technology, Beijing, 100081, PR China
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  • Bin Wei,

    1. School of Life Science and Technology, Beijing Institute of Technology, Beijing, 100081, PR China
    2. School of Chemistry and Chemical Engineering, Shihezi University, Shihezi 832003, Xinjiang, PR China
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  • Xuetong Zhang,

    Corresponding author
    1. School of Materials Science & Engineering, Beijing Institute of Technology, Beijing, 100081, PR China
    • Xuetong Zhang, School of Materials Science & Engineering, Beijing Institute of Technology, Beijing, 100081, PR China

      Chun Li, School of Life Science and Technology, Beijing Institute of Technology, Beijing, 100081, PR China.

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  • Chun Li

    Corresponding author
    1. School of Life Science and Technology, Beijing Institute of Technology, Beijing, 100081, PR China
    • Xuetong Zhang, School of Materials Science & Engineering, Beijing Institute of Technology, Beijing, 100081, PR China

      Chun Li, School of Life Science and Technology, Beijing Institute of Technology, Beijing, 100081, PR China.

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Abstract

Highly porous hosting materials with conducting (favorable to electron transfer) and magnetic (favorable to product separation) bicontinuous networks should possess great potentials for immobilization of various enzymes in the field of biocatalytic engineering, but the synthesis of such materials is still a great challenge. Herein, bifunctional graphene/γ-Fe2O3 hybrid aerogels with quite low density (30–65 mg cm−3), large specific surface area (270–414 m2 g−1), high electrical conductivity (0.5–5 × 10−2 S m−1), and superior saturation magnetization (23–54 emu g−1) are fabricated. Single networks of either graphene aerogels or γ-Fe2O3 aerogels are obtained by etching of the hybrid aerogels with acid solution or calcining of the hybrid aerogels in air, indicative of the double networks of the as-synthesized graphene/γ-Fe2O3 hybrid aerogels for the first time. The resulting bifunctional aerogels are used to immobilize β-glucuronidase for biocatalytic transformation of glycyrrhizin into glycyrrhetinic acid monoglucuronide or glycyrrhetinic acid, with high biocatalytic activity and definite repeatability.

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