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Cell-Laden Hydrogels in Integrated Microfluidic Devices for Long-Term Cell Culture and Tubulogenesis Assays

Authors

  • Nathan P. Gabrielson,

    1. Institute for Genomic Biology, University of Illinois at Urbana-Champaign, 1206 West Gregory Dr. Urbana, IL 61801, USA
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  • Amit V. Desai,

    1. Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, 600 South Mathews Ave, Urbana, IL 61801, USA
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  • Bhushan Mahadik,

    1. Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, 600 South Mathews Ave, Urbana, IL 61801, USA
    2. Institute for Genomic Biology, University of Illinois at Urbana-Champaign, 1206 West Gregory Dr. Urbana, IL 61801, USA
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  • Marie-Claude Hofmann,

    1. Institute for Genomic Biology, University of Illinois at Urbana-Champaign, 1206 West Gregory Dr. Urbana, IL 61801, USA
    2. Department of Comparative Biosciences, University of Illinois at Urbana-Champaign, 2001 South Lincoln Avenue, Urbana, IL 61802, USA
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  • Paul J. A. Kenis,

    1. Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, 600 South Mathews Ave, Urbana, IL 61801, USA
    2. Institute for Genomic Biology, University of Illinois at Urbana-Champaign, 1206 West Gregory Dr. Urbana, IL 61801, USA
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  • Brendan A. C. Harley

    Corresponding author
    1. Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, 600 South Mathews Ave, Urbana, IL 61801, USA
    2. Institute for Genomic Biology, University of Illinois at Urbana-Champaign, 1206 West Gregory Dr. Urbana, IL 61801, USA
    • Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, 600 South Mathews Ave, Urbana, IL 61801, USA.
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Abstract

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A hydrogel biochip combining microfluidic mixing and orthogonal supplementation strategies is developed and validated to allow facile generation of libraries of optically transparent 3D culture microenvironments. Live, on-chip tracing of embryonic stem cell differentiation and endothelial cell tubulogenesis confirms that the platform can be used to both create communities of discrete 3D microenvironments as well as to locally monitor subsequent divergent responses at both single cell and multi-cell scales.

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