Development of Polymeric Nanoprobes with Improved Lifetime Dynamic Range and Stability for Intracellular Oxygen Sensing

Authors

  • Heng Liu,

    1. School of Chemistry and Chemical Engineering, University of Chinese Academy of Sciences (UCAS), Beijing 100049, PR China
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  • Hui Yang,

    1. School of Chemistry and Chemical Engineering, University of Chinese Academy of Sciences (UCAS), Beijing 100049, PR China
    2. Hebei Key Laboratory of Applied Chemistry, School of Environmental and Chemical Engineering, Yanshan University, Qinghuangdao 066004, PR China
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  • Xian Hao,

    1. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences (CAS), Changchun 130022, PR China
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  • Haijiao Xu,

    1. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences (CAS), Changchun 130022, PR China
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  • Yi Lv,

    1. School of Chemistry and Chemical Engineering, University of Chinese Academy of Sciences (UCAS), Beijing 100049, PR China
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  • Debao Xiao,

    Corresponding author
    1. Hebei Key Laboratory of Applied Chemistry, School of Environmental and Chemical Engineering, Yanshan University, Qinghuangdao 066004, PR China
    • Hebei Key Laboratory of Applied Chemistry, School of Environmental and Chemical Engineering, Yanshan University, Qinghuangdao 066004, PR China
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  • Hongda Wang,

    Corresponding author
    1. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences (CAS), Changchun 130022, PR China
    • State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences (CAS), Changchun 130022, PR China.
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  • Zhiyuan Tian

    Corresponding author
    1. School of Chemistry and Chemical Engineering, University of Chinese Academy of Sciences (UCAS), Beijing 100049, PR China
    • School of Chemistry and Chemical Engineering, University of Chinese Academy of Sciences (UCAS), Beijing 100049, PR China
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Abstract

A class of core-shell nanoparticles possessing a layer of biocompatible shell and hydrophobic core with embedded oxygen-sensitive platinum-porphyrin (PtTFPP) dyes is developed via a radical-initiated microemulsion co-polymerization strategy. The influences of host matrices and the PtTFPP incorporation manner on the photophysical properties and the oxygen-sensing performance of the nanoparticles are investigated. Self-loading capability with cells and intracellular-oxygen-sensing ability of the as-prepared nanoparticle probes in the range 0%–20% oxygen concentration are confirmed. Polymeric nanoparticles with optimized formats are characterized by their relatively small diameter (<50 nm), core-shell structures with biocompatible shells, covalent-attachment-imparted leak-free construction, improved lifetime dynamic range (up to 44 μs), excellent storage stability and photostability, and facile cell uptake. The nanoparticles’ small sensor diameter and core-shell structure with biocompatible shell make them suitable for intracellular detection applications. For intracellular detection applications, the leak-free feature of the as-prepared nanoparticle sensor effectively minimizes potential chemical interferences and cytotoxicity. As a salient feature, improved lifetime dynamic range of the sensor is expected to enable precise oxygen detection and control in specific practical applications in stem-cell biology and medical research. Such a feature-packed nanoparticle oxygen sensor may find applications in precise oxygen-level mapping of living cells and tissue.

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