NIR-Emitting Quantum Dot-Encoded Microbeads through Membrane Emulsification for Multiplexed Immunoassays

Authors

  • Xiebing Wang,

    1. State Key Lab of Metal Matrix Composites, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, PR China
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  • Gang Wang,

    1. State Key Lab of Metal Matrix Composites, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, PR China
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  • Wanwan Li,

    Corresponding author
    1. State Key Lab of Metal Matrix Composites, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, PR China
    2. Laboratory of Molecular Imaging and Nanomedicine (LOMIN), National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH), Bethesda, MD 20892, USA
    • State Key Lab of Metal Matrix Composites, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, PR China
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  • Bingxia Zhao,

    1. State Key Lab of Metal Matrix Composites, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, PR China
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  • Bin Xing,

    1. State Key Lab of Metal Matrix Composites, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, PR China
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  • Yuankui Leng,

    1. State Key Lab of Metal Matrix Composites, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, PR China
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  • Hongjing Dou,

    1. State Key Lab of Metal Matrix Composites, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, PR China
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  • Kang Sun,

    Corresponding author
    1. State Key Lab of Metal Matrix Composites, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, PR China
    • State Key Lab of Metal Matrix Composites, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, PR China
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  • Lisong Shen,

    1. Department of Clinical Laboratory, Xinhua Hospital Affiliated to Shanghai Jiao Tong, University School of Medicine, 1665 Kongjiang Road, Shanghai 200092, PR China
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  • Xiangliang Yuan,

    1. Department of Clinical Laboratory, Xinhua Hospital Affiliated to Shanghai Jiao Tong, University School of Medicine, 1665 Kongjiang Road, Shanghai 200092, PR China
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  • Jiyu Li,

    1. Department of Clinical Laboratory, Xinhua Hospital Affiliated to Shanghai Jiao Tong, University School of Medicine, 1665 Kongjiang Road, Shanghai 200092, PR China
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  • Kun Sun,

    1. State Key Lab of Metal Matrix Composites, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, PR China
    2. Department of Clinical Laboratory, Xinhua Hospital Affiliated to Shanghai Jiao Tong, University School of Medicine, 1665 Kongjiang Road, Shanghai 200092, PR China
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  • Junsong Han,

    1. Shanghai Biochip Co., Ltd. & National Engineering, Center for Biochip at Shanghai, 151 Libing Road, Shanghai 201203, PR China
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  • Huasheng Xiao,

    1. Shanghai Biochip Co., Ltd. & National Engineering, Center for Biochip at Shanghai, 151 Libing Road, Shanghai 201203, PR China
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  • Yue Li,

    1. Shanghai Biochip Co., Ltd. & National Engineering, Center for Biochip at Shanghai, 151 Libing Road, Shanghai 201203, PR China
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  • Peng Huang,

    1. Laboratory of Molecular Imaging and Nanomedicine (LOMIN), National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH), Bethesda, MD 20892, USA
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  • Xiaoyuan Chen

    Corresponding author
    1. Laboratory of Molecular Imaging and Nanomedicine (LOMIN), National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH), Bethesda, MD 20892, USA
    • Laboratory of Molecular Imaging and Nanomedicine (LOMIN), National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH), Bethesda, MD 20892, USA.
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Abstract

NIR-emitting CdSeTe/CdS/ZnS core/shell/shell QD-encoded microbeads are combined with common flow cytometry with one laser for multiplexed detection of hepatitis B virus (HBV). A facile one-pot synthetic route is developed to prepare CdSeTe/CdS/ZnS core/shell/shell QDs with high photoluminescence quantum yield and excellent stability in liquid paraffin, and a Shirasu porous glass (SPG) membrane emulsification technique is applied to incorporate the QDs into polystyrene–maleic anhydride (PSMA) microbeads to obtain highly fluorescent QD-encoded microbeads. The relatively wide NIR photoluminescence full width half maximum of the CdSeTe/CdS/ZnS QDs is used to develop a ‘single wavelength’ encoding method to obtain different optical codes by changing the wavelengh and emission intensity of the QDs incorporated into the microbeads. Moreover, a detection platform combining NIR-emitting CdSeTe/CdS/ZnS QD-encoded microbeads and Beckman Coulter FC 500 flow cytometry with one laser of 488 nm is successfully used to conduct a 2-plex hybridization assay for hepatitis B surface antigen (HBsAg), hepatitis B e antigen (HBeAg), and a 3-plex hybridization assay for hepatitis B surface antibody (HBsAb), hepatitis B e antibody (HBeAb), and hepatitis B core antibody (HBcAb), which suggests the promising application of NIR QD-encoded microbeads for multiplex immunoassays.

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