Affinity Separation: M13 Bacteriophage-Activated Superparamagnetic Beads for Affinity Separation (Small 15/2012)

Authors

  • Julien Muzard,

    1. UCD Centre for Nanomedicine, School of Chemistry & Chemical Biology - University, College Dublin Belfield, Dublin 4, Ireland
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  • Mark Platt,

    1. UCD Centre for Nanomedicine, School of Chemistry & Chemical Biology - University, College Dublin Belfield, Dublin 4, Ireland
    Current affiliation:
    1. Analytical Chemistry Department, Loughborough University, UK.
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  • Gil U. Lee

    Corresponding author
    1. UCD Centre for Nanomedicine, School of Chemistry & Chemical Biology - University, College Dublin Belfield, Dublin 4, Ireland
    • UCD Centre for Nanomedicine, School of Chemistry & Chemical Biology - University, College Dublin Belfield, Dublin 4, Ireland.
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Abstract

original image

M13 bacteriophage virons are immobilized on superparamagnetic beads by G. U. Lee and co-workers, either through the pIII proteins on the ends of the phages or by the pVIII proteins on the sides of the phages. These two immobilization schemes, shown in detail on page 2403, result in an end-on phage–superparamagnetic bead assembly or a monolayer of phage covering the superparamagnetic beads in a side-on orientation, respectively.

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