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Enzyme Catalysis: Enzymatic Growth of Quantum Dots: Applications to Probe Glucose Oxidase and Horseradish Peroxidase and Sense Glucose (Small 22/2012)

Authors

  • Laura Saa,

    1. CIC biomaGUNE, Parque Tecnológico de San Sebastián, Paseo Miramón 182, 20009, San Sebastián, Spain, Tel. +34943005308; Fax +34943005314
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  • Valeri Pavlov

    Corresponding author
    1. CIC biomaGUNE, Parque Tecnológico de San Sebastián, Paseo Miramón 182, 20009, San Sebastián, Spain, Tel. +34943005308; Fax +34943005314
    • CIC biomaGUNE, Parque Tecnológico de San Sebastián, Paseo Miramón 182, 20009, San Sebastián, Spain, Tel. +34943005308; Fax +34943005314.
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Abstract

original image

Three innovative assays have been developed for the detection of the enzymatic activities of glucose oxidase (GOx) and horseradish peroxidase (HRP) by the generation of CdS quantum dots (QDs) in situ using an unconventional enzymatic reaction. By employing 1-thiob-D-glucose and sodium thiosulfate as enzymatic substrates, sulfide ions are generated which, in the presence of cadmium ions, yield fluorescent CdS nanoparticles. On page 3449, L. Saa and V. Pavlov show how the combination of GOx with HRP allows quantification of the glucose in human plasma by following the growth of the fluorescent QDs.

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