Ammonium and Guanidinium Dendron–Carbon Nanotubes by Amidation and Click Chemistry and their Use for siRNA Delivery
Article first published online: 6 MAY 2013
© 2013 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. The copyright line for this article was changed on 16 Sept 2014 after original online publication.
Volume 9, Issue 21, pages 3610–3619, November 11, 2013
How to Cite
Battigelli, A., Wang, J. T.-W., Russier, J., Da Ros, T., Kostarelos, K., Al-Jamal, K. T., Prato, M. and Bianco, A. (2013), Ammonium and Guanidinium Dendron–Carbon Nanotubes by Amidation and Click Chemistry and their Use for siRNA Delivery. Small, 9: 3610–3619. doi: 10.1002/smll.201300264
- Issue published online: 4 NOV 2013
- Article first published online: 6 MAY 2013
- Manuscript Revised: 20 FEB 2013
- Manuscript Received: 24 JAN 2013
- Funded Access
- carbon nanotubes;
- cationic dendrons;
- RNA delivery;
- gene therapy
A series of multi-walled carbon nanotube (MWCNT) conjugates is described, functionalized with different dendrons bearing positive charges at their termini (i.e. ammonium or guanidinium groups). The dendrimeric units are anchored to the nanotube scaffolds using two orthogonal synthetic approaches, amidation and click reactions. The final nanohybrids are characterized by complementary analytical techniques, while their ability to interact with siRNA is investigated by means of agarose gel electrophoresis. The demonstration of the cell uptake capacity, the low cytotoxicity, and the ability of these cationic conjugates to silence cytotoxic genes suggests them to be promising carriers for genetic material.