• Open Access

Ammonium and Guanidinium Dendron–Carbon Nanotubes by Amidation and Click Chemistry and their Use for siRNA Delivery

Authors

  • Alessia Battigelli,

    1. CNRS, Institut de Biologie Moléculaire et Cellulaire, Laboratoire d'Immunopathologie, et Chimie Thérapeutique, 67000 Strasbourg, France
    2. Dipartimento di Scienze Chimiche e Farmaceutiche, Università di Trieste 34127, Trieste, Italy
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  • Julie Tzu-Wen Wang,

    1. Drug Delivery Group, Institute of Pharmaceutical Science, King's College London, London SE1 9NH, UK
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  • Julie Russier,

    1. CNRS, Institut de Biologie Moléculaire et Cellulaire, Laboratoire d'Immunopathologie, et Chimie Thérapeutique, 67000 Strasbourg, France
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  • Tatiana Da Ros,

    1. Dipartimento di Scienze Chimiche e Farmaceutiche, Università di Trieste 34127, Trieste, Italy
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  • Kostas Kostarelos,

    1. Nanomedicine Laboratory, UCL School of Pharmacy, University College London, London WC1N 1AX, UK
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  • Khuloud T. Al-Jamal,

    1. Drug Delivery Group, Institute of Pharmaceutical Science, King's College London, London SE1 9NH, UK
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  • Maurizio Prato,

    Corresponding author
    1. Dipartimento di Scienze Chimiche e Farmaceutiche, Università di Trieste 34127, Trieste, Italy
    • Maurizio Prato, Dipartimento di Scienze Chimiche e Farmaceutiche, Università di Trieste 34127, Trieste, Italy.

      Alberto Bianco, CNRS, Institut de Biologie Moléculaire et Cellulaire, Laboratoire d'Immunopathologie, et Chimie Thérapeutique, 67000 Strasbourg, France

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  • Alberto Bianco

    Corresponding author
    1. CNRS, Institut de Biologie Moléculaire et Cellulaire, Laboratoire d'Immunopathologie, et Chimie Thérapeutique, 67000 Strasbourg, France
    • Maurizio Prato, Dipartimento di Scienze Chimiche e Farmaceutiche, Università di Trieste 34127, Trieste, Italy.

      Alberto Bianco, CNRS, Institut de Biologie Moléculaire et Cellulaire, Laboratoire d'Immunopathologie, et Chimie Thérapeutique, 67000 Strasbourg, France

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Abstract

A series of multi-walled carbon nanotube (MWCNT) conjugates is described, functionalized with different dendrons bearing positive charges at their termini (i.e. ammonium or guanidinium groups). The dendrimeric units are anchored to the nanotube scaffolds using two orthogonal synthetic approaches, amidation and click reactions. The final nanohybrids are characterized by complementary analytical techniques, while their ability to interact with siRNA is investigated by means of agarose gel electrophoresis. The demonstration of the cell uptake capacity, the low cytotoxicity, and the ability of these cationic conjugates to silence cytotoxic genes suggests them to be promising carriers for genetic material.

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