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Use of Coated Silver Nanoparticles to Understand the Relationship of Particle Dissolution and Bioavailability to Cell and Lung Toxicological Potential

Authors

  • Xiang Wang,

    1. Center for Environmental Implications of Nanotechnology, California NanoSystems Institute, University of California at Los Angeles, Los Angeles, CA, USA
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  • Zhaoxia Ji,

    1. Center for Environmental Implications of Nanotechnology, California NanoSystems Institute, University of California at Los Angeles, Los Angeles, CA, USA
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  • Chong Hyun Chang,

    1. Center for Environmental Implications of Nanotechnology, California NanoSystems Institute, University of California at Los Angeles, Los Angeles, CA, USA
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  • Haiyuan Zhang,

    1. Center for Environmental Implications of Nanotechnology, California NanoSystems Institute, University of California at Los Angeles, Los Angeles, CA, USA
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  • Meiying Wang,

    1. Center for Environmental Implications of Nanotechnology, California NanoSystems Institute, University of California at Los Angeles, Los Angeles, CA, USA
    2. Division of NanoMedicine, Department of Medicine, University of California at Los Angeles, Los Angeles, CA, USA
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  • Yu-Pei Liao,

    1. Center for Environmental Implications of Nanotechnology, California NanoSystems Institute, University of California at Los Angeles, Los Angeles, CA, USA
    2. Division of NanoMedicine, Department of Medicine, University of California at Los Angeles, Los Angeles, CA, USA
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  • Sijie Lin,

    1. Center for Environmental Implications of Nanotechnology, California NanoSystems Institute, University of California at Los Angeles, Los Angeles, CA, USA
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  • Huan Meng,

    1. Center for Environmental Implications of Nanotechnology, California NanoSystems Institute, University of California at Los Angeles, Los Angeles, CA, USA
    2. Division of NanoMedicine, Department of Medicine, University of California at Los Angeles, Los Angeles, CA, USA
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  • Ruibin Li,

    1. Center for Environmental Implications of Nanotechnology, California NanoSystems Institute, University of California at Los Angeles, Los Angeles, CA, USA
    2. Division of NanoMedicine, Department of Medicine, University of California at Los Angeles, Los Angeles, CA, USA
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  • Bingbing Sun,

    1. Center for Environmental Implications of Nanotechnology, California NanoSystems Institute, University of California at Los Angeles, Los Angeles, CA, USA
    2. Division of NanoMedicine, Department of Medicine, University of California at Los Angeles, Los Angeles, CA, USA
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  • Laura Van Winkle,

    1. Department of Anatomy, Physiology and Cell Biology, School of Veterinary Medicine, University of California at Davis, Davis, CA, USA
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  • Kent E. Pinkerton,

    1. Center for Health and the Environmen, University of California at Davis, Davis, CA, USA
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  • Jeffrey I. Zink,

    1. Center for Environmental Implications of Nanotechnology, California NanoSystems Institute, University of California at Los Angeles, Los Angeles, CA, USA
    2. Department of Chemistry and Biochemistry, University of California, Los Angeles, CA, USA
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  • Tian Xia,

    1. Center for Environmental Implications of Nanotechnology, California NanoSystems Institute, University of California at Los Angeles, Los Angeles, CA, USA
    2. Division of NanoMedicine, Department of Medicine, University of California at Los Angeles, Los Angeles, CA, USA
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  • André E. Nel

    Corresponding author
    1. Center for Environmental Implications of Nanotechnology, California NanoSystems Institute, University of California at Los Angeles, Los Angeles, CA, USA
    2. Division of NanoMedicine, Department of Medicine, University of California at Los Angeles, Los Angeles, CA, USA
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Abstract

Since more than 30% of consumer products that include engineered nanomaterials contain nano-Ag, the safety of this material is of considerable public concern. In this study, Ag nanoparticles (NPs) are used to demonstrate that 20 nm polyvinylpyrrolidone (PVP or P) and citrate (C)-coated Ag NPs induce more cellular toxicity and oxidative stress than larger (110 nm) particles due to a higher rate of dissolution and Ag bioavailability. Moreover, there is also a higher propensity for citrate 20 nm (C20) nanoparticles to generate acute neutrophilic inflammation in the lung and to produce chemokines compared to C110. P110 has less cytotoxic effects than C110, likely due to the ability of PVP to complex released Ag+. In contrast to the more intense acute pulmonary effects of C20, C110 induces mild pulmonary fibrosis at day 21, likely as a result of slow but persistent Ag+ release leading to a sub-chronic injury response. Interestingly, the released metallic Ag is incorporated into the collagen fibers depositing around airways and the lung interstitium. Taken together, these results demonstrate that size and surface coating affect the cellular toxicity of Ag NPs as well as their acute versus sub-chronic lung injury potential.

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