3D Cell Cultures: Nanostructured Superhydrophobic Substrates Trigger the Development of 3D Neuronal Networks (Small 3/2013)

Authors

  • Tania Limongi,

    1. Department of Nanostructures, Istituto Italiano di Tecnologia, Via Morego 30 - 16163 Genova, Italy
    2. Department of Neuroscience and Brain Technologies, Istituto Italiano di Tecnologia, Via Morego 30 - 16163 Genova, Italy
    Current affiliation:
    1. These authors share first authorship.
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  • Fabrizia Cesca,

    1. Department of Neuroscience and Brain Technologies, Istituto Italiano di Tecnologia, Via Morego 30 - 16163 Genova, Italy
    Current affiliation:
    1. These authors share first authorship.
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  • Francesco Gentile,

    1. Department of Nanostructures, Istituto Italiano di Tecnologia, Via Morego 30 - 16163 Genova, Italy
    2. Department of Experimental and Clinical Medicine, BioNEM Laboratory, University Magna Grecia of Catanzaro, Viale Europa- 88100 Catanzaro, Italy
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  • Roberto Marotta,

    1. Department of Nanochemistry, Istituto Italiano di Tecnologia, Via Morego 30 - 16163 Genova, Italy
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  • Roberta Ruffilli,

    1. Department of Nanochemistry, Istituto Italiano di Tecnologia, Via Morego 30 - 16163 Genova, Italy
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  • Andrea Barberis,

    1. Department of Neuroscience and Brain Technologies, Istituto Italiano di Tecnologia, Via Morego 30 - 16163 Genova, Italy
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  • Marco Dal Maschio,

    1. Department of Neuroscience and Brain Technologies, Istituto Italiano di Tecnologia, Via Morego 30 - 16163 Genova, Italy
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  • Enrica Maria Petrini,

    1. Department of Neuroscience and Brain Technologies, Istituto Italiano di Tecnologia, Via Morego 30 - 16163 Genova, Italy
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  • Stefania Santoriello,

    1. Department of Nanostructures, Istituto Italiano di Tecnologia, Via Morego 30 - 16163 Genova, Italy
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  • Fabio Benfenati,

    1. Department of Neuroscience and Brain Technologies, Istituto Italiano di Tecnologia, Via Morego 30 - 16163 Genova, Italy
    Current affiliation:
    1. These authors share last authorship.
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  • Enzo Di Fabrizio

    Corresponding author
    1. Department of Nanostructures, Istituto Italiano di Tecnologia, Via Morego 30 - 16163 Genova, Italy
    Current affiliation:
    1. These authors share last authorship.
    • Department of Nanostructures, Istituto Italiano di Tecnologia, Via Morego 30 - 16163 Genova, Italy.
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Abstract

original image

On page 402, E. Di Fabrizio and co-workers describe the growth of neuron–astrocyte co-cultures on nanostructured superhydrophobic substrates. The scanning electron microscopy image on the inside cover shows a flat glial cell monolayer (pink) suspended between adjacent nanostructured pillars. Astrocytes adhere with high affinity to both the top and lateral surface of the pillars and provide an excellent support for cocultured neurons, as shown by a large neuron (blue) spreading upon the glial carpet with its multiple neuritic processes.

Ancillary