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Microfluidics: Three Dimensional, Sheathless, and High-Throughput Microparticle Inertial Focusing Through Geometry-Induced Secondary Flows (Small 5/2013)

Authors

  • Aram J. Chung,

    1. Department of Bioengineering, California NanoSystems Institute, University of California, Los Angeles, CA 90095, USA, Phone: (310) 983-3235, Fax: (310) 794-5956
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  • Daniel R. Gossett,

    1. Department of Bioengineering, California NanoSystems Institute, University of California, Los Angeles, CA 90095, USA, Phone: (310) 983-3235, Fax: (310) 794-5956
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  • Dino Di Carlo

    Corresponding author
    1. Department of Bioengineering, California NanoSystems Institute, University of California, Los Angeles, CA 90095, USA, Phone: (310) 983-3235, Fax: (310) 794-5956
    • Department of Bioengineering, California NanoSystems Institute, University of California, Los Angeles, CA 90095, USA, Phone: (310) 983-3235, Fax: (310) 794-5956.
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Abstract

original image

The process of single-stream microparticle inertial focusing in a single focal plane without sheath fluids and external forces, all in a high-throughput manner, is described on page 685 by D. Di Carlo and co-workers. The proposed design consists of a low-aspect-ratio straight channel interspersed with a series of constrictions in height arranged orthogonally, making use of two inertial effects: inertial microparticle focusing and geometry-induced secondary flows. A focusing efficiency as high as 99.77% is demonstrated, with a throughput as high as 36 000 particles s−1 for a variety of different sized particles and cells. Cover illustration by Marc Lim.

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