Gold Nanodots: In vivo Metabolic Imaging of Insulin with Multiphoton Fluorescence of Human Insulin–Au Nanodots (Small 12/2013)

Authors

  • Chien-Liang Liu,

    1. Department of Chemistry, National Taiwan University, Taipei, 10617 Taiwan, Fax: +886-2-369-5208
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  • Tzu-Ming Liu,

    Corresponding author
    1. Institute of Biomedical Engineering, National Taiwan University, Taipei 10617, Taiwan, Fax: +886-2-2394-0049
    2. Molecular Imaging Center, National Taiwan University, Taipei 10617, Taiwan
    • Institute of Biomedical Engineering, National Taiwan University, Taipei 10617, Taiwan, Fax: +886-2-2394-0049.
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  • Tsung-Yuan Hsieh,

    1. Institute of Biomedical Engineering, National Taiwan University, Taipei 10617, Taiwan, Fax: +886-2-2394-0049
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  • Han-Wen Liu,

    1. Institute of Biomedical Engineering, National Taiwan University, Taipei 10617, Taiwan, Fax: +886-2-2394-0049
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  • Yu-Shing Chen,

    1. Institute of Biomedical Engineering, National Taiwan University, Taipei 10617, Taiwan, Fax: +886-2-2394-0049
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  • Cheng-Kun Tsai,

    1. Institute of Biomedical Engineering, National Taiwan University, Taipei 10617, Taiwan, Fax: +886-2-2394-0049
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  • Hsieh-Chih Chen,

    1. Department of Chemistry, National Taiwan University, Taipei, 10617 Taiwan, Fax: +886-2-369-5208
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  • Jong-Wei Lin,

    1. Division of Cardiology, Department of Internal Medicine, National Taiwan University Hospital, National Taiwan University College of Medicine, Taipei 10002, Taiwan
    2. Cardiovascular Center and Department of Internal Medicine, National Taiwan University Hospital Yun-Lin Branch, Yun-Lin county 64041, Taiwan
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  • Ron-Bin Hsu,

    1. Department of Surgery, National Taiwan University Hospital and National Taiwan University College of Medicine, Taipei 10002, Taiwan
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  • Tzung-Dau Wang,

    1. Division of Cardiology, Department of Internal Medicine, National Taiwan University Hospital, National Taiwan University College of Medicine, Taipei 10002, Taiwan
    2. Cardiovascular Center and Department of Internal Medicine, National Taiwan University Hospital Yun-Lin Branch, Yun-Lin county 64041, Taiwan
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  • Chien-Cheng Chen,

    1. Graduate Institute of Photonics and Optoelectronics, National Taiwan University, Taipei 10617, Taiwan
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  • Chi-Kuang Sun,

    1. Molecular Imaging Center, National Taiwan University, Taipei 10617, Taiwan
    2. Graduate Institute of Photonics and Optoelectronics, National Taiwan University, Taipei 10617, Taiwan
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  • Pi-Tai Chou

    Corresponding author
    1. Department of Chemistry, National Taiwan University, Taipei, 10617 Taiwan, Fax: +886-2-369-5208
    • Department of Chemistry, National Taiwan University, Taipei, 10617 Taiwan, Fax: +886-2-369-5208
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Abstract

original image

In vivo imaging of insulin metabolism is realized by T.-M. Liu, P.-T. Chou and co-workers using synthesized human insulin–Au nanodots (NDs). Benefiting from its efficient red to near infrared emission, deep tissue subcellular uptake of insulin–Au NDs can be evaluated with a nonlinear optical microscope. On page 2103, insulin-Au NDs permeate into lipid droplets (LDs) of adipocytes, and enlarged ones in type II diabetes mice have a higher adjacent/LD concentration contrast. Human clinical samples show similar trends in patients with diabetes and coronary artery diseas

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