Cancer Diagnostics: A Trachea-Inspired Bifurcated Microfilter Capturing Viable Circulating Tumor Cells via Altered Biophysical Properties as Measured by Atomic Force Microscopy (Small 18/2013)

Authors

  • Minseok S. Kim,

    Corresponding author
    1. POCT In Vitro Diagnostics Group, R&D Solution Group, Samsung Advanced Institute of Technology (SAIT), San 14, Nongseo-dong, Giheung-gu, Yongin-si, Gyeonggi-do, Korea
    • POCT In Vitro Diagnostics Group, R&D Solution Group, Samsung Advanced Institute of Technology (SAIT), San 14, Nongseo-dong, Giheung-gu, Yongin-si, Gyeonggi-do, Korea.
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  • Jinhoon Kim,

    1. POCT In Vitro Diagnostics Group, R&D Solution Group, Samsung Advanced Institute of Technology (SAIT), San 14, Nongseo-dong, Giheung-gu, Yongin-si, Gyeonggi-do, Korea
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  • Wonho Lee,

    1. POCT In Vitro Diagnostics Group, R&D Solution Group, Samsung Advanced Institute of Technology (SAIT), San 14, Nongseo-dong, Giheung-gu, Yongin-si, Gyeonggi-do, Korea
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  • Sang-Joon Cho,

    1. Research and Development Center, Park Systems, Korea
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  • Jin-Mi Oh,

    1. POCT In Vitro Diagnostics Group, R&D Solution Group, Samsung Advanced Institute of Technology (SAIT), San 14, Nongseo-dong, Giheung-gu, Yongin-si, Gyeonggi-do, Korea
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  • June-Young Lee,

    1. POCT In Vitro Diagnostics Group, R&D Solution Group, Samsung Advanced Institute of Technology (SAIT), San 14, Nongseo-dong, Giheung-gu, Yongin-si, Gyeonggi-do, Korea
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  • Sanghyun Baek,

    1. POCT In Vitro Diagnostics Group, R&D Solution Group, Samsung Advanced Institute of Technology (SAIT), San 14, Nongseo-dong, Giheung-gu, Yongin-si, Gyeonggi-do, Korea
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  • Yeon Jeong Kim,

    1. POCT In Vitro Diagnostics Group, R&D Solution Group, Samsung Advanced Institute of Technology (SAIT), San 14, Nongseo-dong, Giheung-gu, Yongin-si, Gyeonggi-do, Korea
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  • Tae Seok Sim,

    1. POCT In Vitro Diagnostics Group, R&D Solution Group, Samsung Advanced Institute of Technology (SAIT), San 14, Nongseo-dong, Giheung-gu, Yongin-si, Gyeonggi-do, Korea
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  • Hun Joo Lee,

    1. POCT In Vitro Diagnostics Group, R&D Solution Group, Samsung Advanced Institute of Technology (SAIT), San 14, Nongseo-dong, Giheung-gu, Yongin-si, Gyeonggi-do, Korea
    2. Interdisciplinary Program of Integrated, Biotechnology, Sogang University, Korea
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  • Goo-Eun Jung,

    1. Research and Development Center, Park Systems, Korea
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  • Seung-Il Kim,

    1. Department of Surgery, Yonsei University College of Medicine, Korea
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  • Jong-Myeon Park,

    1. POCT In Vitro Diagnostics Group, R&D Solution Group, Samsung Advanced Institute of Technology (SAIT), San 14, Nongseo-dong, Giheung-gu, Yongin-si, Gyeonggi-do, Korea
    2. Department of Chemistry and Center for Bioactive, Molecular Hybrids, Yonsei University, Korea
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  • Jin Ho Oh,

    1. POCT In Vitro Diagnostics Group, R&D Solution Group, Samsung Advanced Institute of Technology (SAIT), San 14, Nongseo-dong, Giheung-gu, Yongin-si, Gyeonggi-do, Korea
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  • Ogan Gurel,

    1. MOT Research Center, CTO Office, Samsung Advanced Institute of Technology, Korea and Samsung Advanced Institute of Health Sciences & Technology, SungKyunKwan University, Korea
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  • Soo Suk Lee,

    Corresponding author
    1. POCT In Vitro Diagnostics Group, R&D Solution Group, Samsung Advanced Institute of Technology (SAIT), San 14, Nongseo-dong, Giheung-gu, Yongin-si, Gyeonggi-do, Korea
    • POCT In Vitro Diagnostics Group, R&D Solution Group, Samsung Advanced Institute of Technology (SAIT), San 14, Nongseo-dong, Giheung-gu, Yongin-si, Gyeonggi-do, Korea.
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  • Jeong-Gun Lee

    Corresponding author
    1. POCT In Vitro Diagnostics Group, R&D Solution Group, Samsung Advanced Institute of Technology (SAIT), San 14, Nongseo-dong, Giheung-gu, Yongin-si, Gyeonggi-do, Korea
    • POCT In Vitro Diagnostics Group, R&D Solution Group, Samsung Advanced Institute of Technology (SAIT), San 14, Nongseo-dong, Giheung-gu, Yongin-si, Gyeonggi-do, Korea.
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Abstract

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In isolating circulating tumor cells (CTCs), which exist in extremely low concentrations in the blood, current methods have been limited by a trade-off between recovery rate and purity. To overcome this challenge, M. S. Kim, S. S. Lee, J.-G. Lee, and co-workers present on page 3103 a sized-based filtration method that combines two strategies: (1) using specific binding of solid microbeads conjugated with anti-EpCAM antibodies to enhance the discrimination of CTCs from other blood constituents (mainly white blood cells) along with (2) a novel bio-inspired bifurcated microfilter that minimizes fluidic stresses on these cells, once captured. The method approaches maximal recovery rate and purity, while allowing viable CTCs to be isolated, enabling not only their enumeration but also further molecular and cellular evaluation.

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