Microfluidic Sorting: Exhaustion of Racing Sperm in Nature-Mimicking Microfluidic Channels During Sorting (Small 20/2013)

Authors

  • Savas Tasoglu,

    1. Bio-Acoustic-MEMS in Medicine (BAMM) Laboratory, Center for Bioengineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA
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  • Hooman Safaee,

    1. Bio-Acoustic-MEMS in Medicine (BAMM) Laboratory, Center for Bioengineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA
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  • Xiaohui Zhang,

    1. Bio-Acoustic-MEMS in Medicine (BAMM) Laboratory, Center for Bioengineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA
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  • James L. Kingsley,

    1. Department of Physics, Worcester Polytechnic Institute, Worcester, MA, USA
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  • Paolo N. Catalano,

    1. Bio-Acoustic-MEMS in Medicine (BAMM) Laboratory, Center for Bioengineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA
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  • Umut Atakan Gurkan,

    1. Bio-Acoustic-MEMS in Medicine (BAMM) Laboratory, Center for Bioengineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA
    Current affiliation:
    1. Case Biomanufacturing and Microfabrication Laboratory, Mechanical and Aerospace Engineering, Case Western Reserve University, Advanced Platform Technology Center, Louis Stokes Cleveland Veterans Affairs Medical Center, Cleveland, OH, USA
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  • Aida Nureddin,

    1. Bio-Acoustic-MEMS in Medicine (BAMM) Laboratory, Center for Bioengineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA
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  • Emre Kayaalp,

    1. Jamaica Hospital Medical Center, Department of Obstetrics and Gynecology, Queens, NY, USA
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  • Raymond M. Anchan,

    1. Center for Infertility and Reproductive Surgery, Obstetrics Gynecology and Reproductive Biology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA
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  • Richard L. Maas,

    1. Division of Genetics, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA
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  • Erkan Tüzel,

    Corresponding author
    1. Department of Physics, Biomedical Engineering and Computer Science, Worcester Polytechnic Institute, Worcester, MA, USA
    • Erkan Tüzel, Department of Physics, Biomedical Engineering and Computer Science, Worcester Polytechnic Institute, Worcester, MA, USA.

      Utkan Demirci, Bio-Acoustic-MEMS in Medicine (BAMM) Laboratory, Center for Bioengineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA

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  • Utkan Demirci

    Corresponding author
    1. Bio-Acoustic-MEMS in Medicine (BAMM) Laboratory, Center for Bioengineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA
    2. Harvard-Massachusetts Institute of Technology Health Sciences and Technology, Cambridge, MA, USA
    • Erkan Tüzel, Department of Physics, Biomedical Engineering and Computer Science, Worcester Polytechnic Institute, Worcester, MA, USA.

      Utkan Demirci, Bio-Acoustic-MEMS in Medicine (BAMM) Laboratory, Center for Bioengineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA

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Abstract

original image

A simple and cost-effective microfluidic design at the scale of the channels in which sperm swim towards an egg is reported. In these channels, human and mouse sperm movement is quantitatively investigated. As described on page 3374 by E. Tüzel, U. Demirci, and co-workers, a significant role is discovered for mouse sperm exhaustion using experiments and coarse-grained computational modeling. The experimental results are recapitulated by the computational model when mouse sperm is modeled with an average exhaustion time. On the other hand, exhaustion does not play a significant role in human sperm sorting for up to 1 h incubation. The presented platform is broadly applicable to multiple areas including reproductive medicine, veterinary sciences, cryobiology, biobanking, wild life preservation, and cell-taxis studies.

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