Cellular Uptake: Uptake Kinetics and Nanotoxicity of Silica Nanoparticles Are Cell Type Dependent (Small 23/2013)

Authors

  • Julia Blechinger,

    1. Ludwig-Maximilians-University Munich, Department of Chemistry and Center for NanoScience, Butenandtstr.11, Gerhard-Ertl-Gebäude, 81377 Munich, Germany
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  • Alexander T. Bauer,

    1. Experimental Dermatology, Medical Faculty Mannheim, Heidelberg University, Theodor-Kutzer-Ufer 1-3, 68167 Mannheim, Germany
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  • Adriano A. Torrano,

    1. Ludwig-Maximilians-University Munich, Department of Chemistry and Center for NanoScience, Butenandtstr.11, Gerhard-Ertl-Gebäude, 81377 Munich, Germany
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  • Christian Gorzelanny,

    1. Experimental Dermatology, Medical Faculty Mannheim, Heidelberg University, Theodor-Kutzer-Ufer 1-3, 68167 Mannheim, Germany
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  • Christoph Bräuchle,

    Corresponding author
    1. Ludwig-Maximilians-University Munich, Department of Chemistry and Center for NanoScience, Butenandtstr.11, Gerhard-Ertl-Gebäude, 81377 Munich, Germany
    • Christoph Bräuchle, Ludwig-Maximilians-University Munich, Department of Chemistry and Center for NanoScience, Butenandtstr.11, Gerhard-Ertl-Gebäude, 81377 Munich, Germany

      Stefan W. Schneider, Experimental Dermatology, Medical Faculty Mannheim, Heidelberg University, Theodor-Kutzer-Ufer 1-3, 68167 Mannheim, Germany.

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  • Stefan W. Schneider

    Corresponding author
    1. Experimental Dermatology, Medical Faculty Mannheim, Heidelberg University, Theodor-Kutzer-Ufer 1-3, 68167 Mannheim, Germany
    • Christoph Bräuchle, Ludwig-Maximilians-University Munich, Department of Chemistry and Center for NanoScience, Butenandtstr.11, Gerhard-Ertl-Gebäude, 81377 Munich, Germany

      Stefan W. Schneider, Experimental Dermatology, Medical Faculty Mannheim, Heidelberg University, Theodor-Kutzer-Ufer 1-3, 68167 Mannheim, Germany.

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Abstract

original image

Three-dimensional atomic force microscopy is combined with fluorescence microscopy to investigate the cellular uptake of silica nanoparticles into human cells. As reported on page 3970 by C. Bräuchle, S. W. Schneider, and co-workers, nanoparticles (red dots in the image) are visible on the plasma membrane outside of the cells. Interestingly, after longer exposure times, the surface of the cells may also be characterized by homogeneous distribution of small humps, indicating intracellular localization of nanoparticles. Results obtained using this technique confirm that the cellular response to silica nanoparticles is cell type dependent.

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