Polymersomes: Third-Party ATP Sensing in Polymersomes: A Label-Free Assay of Enzyme Reactions in Vesicular Compartments (Small 3/2014)

Authors

  • Umit Hakan Yildiz,

    1. Centre for Biomimetic Sensor Science, School of Materials Science & Engineering, Nanyang Technological University, Singapore
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  • Hans-Peter M. De Hoog,

    1. Centre for Biomimetic Sensor Science, School of Materials Science & Engineering, Nanyang Technological University, Singapore
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  • Zhikang Fu,

    1. Institute of Materials Research and Engineering, Singapore
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  • Nikodem Tomczak,

    1. Institute of Materials Research and Engineering, Singapore
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  • Atul N. Parikh,

    1. Centre for Biomimetic Sensor Science, School of Materials Science & Engineering, Nanyang Technological University, Singapore
    2. Biomedical Engineering, Chemical Engineering and Materials Science, University of California, Davis One Shields Avenue, Davis, CA, USA
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  • Madhavan Nallani,

    1. Centre for Biomimetic Sensor Science, School of Materials Science & Engineering, Nanyang Technological University, Singapore
    2. Institute of Materials Research and Engineering, Singapore
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  • Bo Liedberg

    Corresponding author
    1. Centre for Biomimetic Sensor Science, School of Materials Science & Engineering, Nanyang Technological University, Singapore
    • Centre for Biomimetic Sensor Science, School of Materials Science & Engineering, Nanyang Technological University, 50 Nanyang Drive, Singapore 6375532

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Abstract

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Polymersomes are encapsulated with a fluorescent reporter and a non-labeled enzyme for sensing of adenosine triphosphate (ATP). As B. Liedberg and co-workers report on page 442, passive diffusion of exogenously added ATP through the membrane is sensed by monitoring the ATPinduced fluorescence quenching of the reporter polymer followed by partial recovery of its emission due to hydrolysis of reporter-bound ATP by alkaline phosphatase.

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