Design of a Microchannel-Nanochannel-Microchannel Array Based Nanoelectroporation System for Precise Gene Transfection (pages 1015–1023)
Keliang Gao, Lei Li, Lingna He, Kevin Hinkle, Yun Wu, Junyu Ma, Lingqian Chang, Xi Zhao, Daniel Gallego Perez, Sigrid Eckardt, John Mclaughlin, Boyu Liu, Dave F. Farson and L. James Lee
Article first published online: 31 OCT 2013 | DOI: 10.1002/smll.201300116
A microchannel-nanochannel-microchannel array based nanoelectroporation (NEP) system is designed, fabricated, and applied for precise gene transfection. The fabrication consists of dip combing, Au coating, molecular imprinting, femtosecond laser ablation, packaging and sealing, incorporating with microfluidic loading reservoirs, and spinning for cell loading. Compared with the conventional method, NEP provides relatively high and uniform dosage control for gene transfection.