Atomic Force Microscopy-Derived Nanoscale Chip for the Detection of Human Pathogenic Viruses (pages 847–854)
Helga Artelsmair, Ferry Kienberger, Ali Tinazli, Robert Schlapak, Rong Zhu, Johannes Preiner, Juergen Wruss, Markus Kastner, Nadia Saucedo-Zeni, Martin Hoelzl, Christian Rankl, Werner Baumgartner, Stefan Howorka, Dieter Blaas, Hermann J. Gruber, Robert Tampé and Peter Hinterdorfer
Article first published online: 17 JUN 2008 | DOI: 10.1002/smll.200700691
Using AFM-based native protein nanolithography, nanopatterns of virus receptor molecules are deposited into an anti-adsorptive protein layer (see figure). Single virus particles are then affinity-captured onto the array, as shown by topographical AFM. Both the fabrication of and readout from the nanoscaled chip are conducted under native conditions. The nanoarray of viral receptors appears well-suited for the detection of single virus particles without prelabeling and preamplification.