Nanostructured Superhydrophobic Substrates Trigger the Development of 3D Neuronal Networks (pages 402–412)
Tania Limongi, Fabrizia Cesca, Francesco Gentile, Roberto Marotta, Roberta Ruffilli, Andrea Barberis, Marco Dal Maschio, Enrica Maria Petrini, Stefania Santoriello, Fabio Benfenati and Enzo Di Fabrizio
Article first published online: 2 OCT 2012 | DOI: 10.1002/smll.201201377
A novel method for culturing primary mouse hippocampal neurons and neuron-astrocyte co-cultures using superhydrophobic substrates with characteristic lengths falling into the microscale range and surface patterning in the nano-range is developed. This new culture method fosters neuronal growth and differentiation in an open scaffold, by reducing the interactions with the substrate and maximizing the physiological processes of cell-cell adhesion and synapse formation.