Embryonic Stem Cells/Induced Pluripotent Stem Cells

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miR-290 Cluster Modulates Pluripotency by Repressing Canonical NF-κB Signaling†‡§

  1. Patrick Lüningschrör1,
  2. Benedikt Stöcker1,
  3. Barbara Kaltschmidt1,2,
  4. Christian Kaltschmidt1,¶,*

Article first published online: 22 MAR 2012

DOI: 10.1002/stem.1033

Issue

STEM CELLS

STEM CELLS

Volume 30, Issue 4, pages 655–664, April 2012

Additional Information

How to Cite

Lüningschrör, P., Stöcker, B., Kaltschmidt, B. and Kaltschmidt, C. (2012), miR-290 Cluster Modulates Pluripotency by Repressing Canonical NF-κB Signaling. STEM CELLS, 30: 655–664. doi: 10.1002/stem.1033

Author Information

  1. 1

    Department of Cell Biology, University of Bielefeld, Bielefeld, Germany

  2. 2

    Laboratory of Molecular Neurobiology, University of Bielefeld, Bielefeld, Germany

  1. Telephone: +495211065625; Fax: +495211065654

*Department of Cell Biology, University of Bielefeld, Universitätsstr. 25, 33501 Bielefeld, Germany

  1. †

    Author contributions: P.L.: conception and design, collection and/or assembly of data, data analysis and interpretation, manuscript writing, and final approval of manuscript; B.S.: collection and/or assembly of data, data analysis and interpretation, and final approval of manuscript; B.K.: conception and design, data analysis and interpretation, and final approval of manuscript; C.K.: financial support, conception and design, data analysis and interpretation, manuscript writing, and final approval of manuscript.

  2. ‡

    Disclosure of potential conflicts of interest is found at the end of this article.

  3. §

    First published online in STEM CELLSEXPRESS January 9, 2012.

  4. Telephone: +495211065625; Fax: +495211065654

Publication History

  1. Issue published online: 22 MAR 2012
  2. Article first published online: 22 MAR 2012
  3. Accepted manuscript online: 9 JAN 2012 02:55PM EST
  4. Manuscript Accepted: 19 DEC 2011
  5. Manuscript Received: 15 SEP 2011

Funded by

  • University of Bielefeld and Deutsche Forschungsgemeinschaft (DFG)

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Keywords:

  • NF-κB;
  • Epithelial to mesenchymal transition;
  • Pluripotency;
  • MicroRNA-290 cluster;
  • Embryonic stem cells

Abstract

Embryonic stem cell (ESC)-specific microRNAs (miRNAs) play a critical role in the maintenance of pluripotency and self-renewal but the complete network between these miRNAs and their broad range of target genes still remains elusive. Here we demonstrate that miR-290 cluster, the most abundant miRNA family in ESCs, targets the NF-κB subunit p65 (also known as RelA) by repressing its translation. Forced expression of p65 causes loss of pluripotency, promotes differentiation of ESCs, and leads to an epithelial to mesenchymal transition. These data define p65 as a novel target gene of miR-290 cluster and provide new insight into the function of ESC-specific miRNAs. STEM CELLS2012; 30:655–664

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