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Translational and Clinical Research
Article first published online: 9 APR 2012
Copyright © 2012 AlphaMed Press
Volume 30, Issue 5, pages 1030–1041, May 2012
How to Cite
Eirin, A., Zhu, X.-Y., Krier, J. D., Tang, H., Jordan, K. L., Grande, J. P., Lerman, A., Textor, S. C. and Lerman, L. O. (2012), Adipose Tissue-Derived Mesenchymal Stem Cells Improve Revascularization Outcomes to Restore Renal Function in Swine Atherosclerotic Renal Artery Stenosis. STEM CELLS, 30: 1030–1041. doi: 10.1002/stem.1047
Author contributions: A.E.: conception and design, collection and/or assembly of data, data analysis and interpretation, manuscript writing, and final approval of manuscript; X.Z.: collection and/or assembly of data, data analysis and interpretation, and manuscript writing; J.D.K., H.T., and K.L.J.: collection and/or assembly of data and data analysis and interpretation; J.P.G.: final approval of manuscript; A.L. and S.C.T.: manuscript writing and final approval of manuscript; L.O.L.: conception and design, financial support, collection and/or assembly of data, data analysis and interpretation, manuscript writing, and final approval of manuscript.
Disclosure of potential conflicts of interest is found at the end of this article.
First published online in STEM CELLSEXPRESS January 30, 2012.
- Issue published online: 9 APR 2012
- Article first published online: 9 APR 2012
- Accepted manuscript online: 30 JAN 2012 03:48PM EST
- Manuscript Accepted: 9 JAN 2012
- Manuscript Revised: 23 DEC 2011
- Manuscript Received: 4 OCT 2011
- NIH. Grant Numbers: DK73608, DK77013, HL77131, HL085307, UL1-RR024150
- American Heart Association
Additional Supporting Information may be found in the online version of this article.
|STEM_1047_sm_SuppFig1.tif||1586K||Figure 1s. Schematic of the experimental protocol. RAS: renal artery stenosis, ARAS: atherosclerotic RAS, PTRA: percutaneous transluminal renal angioplasty, MSC: mesenchymal stem cells.|
|STEM_1047_sm_SuppFig2.pdf||671K||Figure 2s. A: Spindle-shaped MSC expanded from adipose tissue. B: Adipose MSC secreted VEGF and TNF-α in culture. Cultured MSC expressed mesenchymal markers (CD44, CD90, and CD105), but not endothelial (CD31) or inflammatory (CD14 and CD45) markers.|
|STEM_1047_sm_SuppFig3.pdf||669K||Figure 3s. Adipose MSC transdifferentiated into osteocytes (Ms xh Osteocalcin), chondrocytes (Gt xh Aggrecan), and adipocytes (Gt x msFABP-4) in vitro (A). Very few fluorescent CM-DiI (arrows, 40x) MSC were observed in the contralateral kidney (B) or myocardium (C) 4 weeks after intra-renal delivery.|
|STEM_1047_sm_SuppFig4.pdf||640K||Figure 4s. Top: Stenotic kidney renal tissue sections stained with proliferating cell nuclear antigen (PCNA) (left) and endothelial cell marker CD31 (right) 4 weeks after MSC administration. Single staining for CM-DiI (arrows, 40×) showing engrafted-MSC (middle) that co-stained with both PCNA and CD31 (bottom).|
|STEM_1047_sm_SuppFig5.pdf||872K||Figure 5s. Treatment with MSC increased the number of proliferating cell nuclear antigen (PCNA, green) positive cells (A) and the staining for the endothelial cell markers CD31 (green) and Von Willebrand factor (vWF, brown) 4 weeks after intra-renal delivery (B). *p<0.05 vs. normal, †p<0.05 vs. ARAS+PTRA+MSC.|
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