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Tissue-Specific Stem Cells
Version of Record online: 9 APR 2012
Copyright © 2012 AlphaMed Press
Volume 30, Issue 5, pages 946–955, May 2012
How to Cite
Xing, Y., Li, A., Borok, Z., Li, C. and Minoo, P. (2012), NOTCH1 Is Required for Regeneration of Clara Cells During Repair of Airway Injury. STEM CELLS, 30: 946–955. doi: 10.1002/stem.1059
Author contributions: Y.X.: conception and design, collection and/or assembly of data, data analysis and interpretation, and manuscript writing; A.L.: collection and/or assembly of data; Z.B.: data analysis and interpretation and manuscript writing; C.L.: data analysis and interpretation; P.M.: conception and design, data analysis and interpretation, manuscript writing, and final approval of manuscript.
Disclosure of potential conflicts of interest is found at the end of this article.
First published online in STEM CELLSEXPRESS February 13, 2012.
- Issue online: 9 APR 2012
- Version of Record online: 9 APR 2012
- Accepted manuscript online: 13 FEB 2012 04:41PM EST
- Manuscript Accepted: 23 JAN 2012
- Manuscript Received: 14 NOV 2011
- Hastings Foundation
Additional Supporting Information may be found in the online version of this article.
|STEM_1059_sm_SuppFig1.pdf||1160K||Supplemental Figure 1. Histology and cell differentiation in Notch1Δ/Δ embryonic lungs. A. Overall morphology and H&E-stained sections of Notch1Δ/Δ and Notch1fl/fl (control) lungs at two embryonic stages. Panels “a” to “d”, E15.5, & Panels “e” to “h”, E18.5 lungs. B. Clara cell differentiation in E18.5 lungs. C. NE, ciliated and alveolar type I cell differentiation in E18.5 lungs (Panels “m” to “r”). Expression of SP-C, SP-B and NKX2.1 in E18.5 lungs (Panels “s” to “x”).|
|STEM_1059_sm_SuppFig2.pdf||329K||Supplemental Figure 2. Histology and cell differentiation in Notch1Δ/Δ adult lung. H&E staining analysis of Notch1fl/fl and Notch1Δ/Δ adult lungs (Panels a & b). Clara and ciliated cell differentiation in Notch1fl/fl and Notch1Δ/Δ adult lungs (Panels c & d).|
|STEM_1059_sm_SuppFig3.pdf||94K||Supplemental Figure 3. Cell differentiation in Notch1Δ/Δ embryonic lungs. CC10, Foxj1, PGP9.5, SpB, SpC and Nkx2.1 in E18.5 Notch1fl/fl and Notch1Δ/Δ lungs were analyzed by real-time PCR.|
|STEM_1059_sm_SuppFig4.tif||424K||Supplemental Figure 4. Colocalization of activated NOTCH1 with markers for Clara or ciliated cells in wild-type E18.5 lungs. Immunolocalization of activated NOTCH1 and CC10 (Panel a) or β-tubulin (Panel b) in wild-type E18.5 lungs. Double positive cells (arrow) and single positive cells (triangle).|
|STEM_1059_sm_SuppFig5.tif||470K||Supplemental Figure 5. PGP9.5positive cells are not ciliated cells in injured and noninjured lungs. Immunolocalization of PGP9.5 and β - tubulin in uninjured lungs (Panels a & c) and lungs after 3 days of naphthalene injury (Panels b & d). PGP9.5positive (red, arrow) and β - tubulinpositive (green, triangle).|
|STEM_1059_sm_SuppFig6.tif||1086K||Supplemental Figure 6. Quantification of cell proliferation after Naphthalene injury. A. Percentage of proliferating cells (Ki67positive) on days 2, 3, 5 and 7 post-injury. Oil was used as injury control. B. Percent proliferating Clara cells (double Ki67positive; CC10positive cells) in airways of control and mutant mice. C. Percent proliferating PGP9.5positive cells (double Phospho-Histone3positive; PGP9.5positive cells) in the airways of mutant and control mice. Cell numbers were determined by manual counting on multiple random fields (n=10).|
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