Additional Supporting Information may be found in the online version of this article.

STEM_1059_sm_SuppFig1.pdf1160KSupplemental Figure 1. Histology and cell differentiation in Notch1Δ/Δ embryonic lungs. A. Overall morphology and H&E-stained sections of Notch1Δ/Δ and Notch1fl/fl (control) lungs at two embryonic stages. Panels “a” to “d”, E15.5, & Panels “e” to “h”, E18.5 lungs. B. Clara cell differentiation in E18.5 lungs. C. NE, ciliated and alveolar type I cell differentiation in E18.5 lungs (Panels “m” to “r”). Expression of SP-C, SP-B and NKX2.1 in E18.5 lungs (Panels “s” to “x”).
STEM_1059_sm_SuppFig2.pdf329KSupplemental Figure 2. Histology and cell differentiation in Notch1Δ/Δ adult lung. H&E staining analysis of Notch1fl/fl and Notch1Δ/Δ adult lungs (Panels a & b). Clara and ciliated cell differentiation in Notch1fl/fl and Notch1Δ/Δ adult lungs (Panels c & d).
STEM_1059_sm_SuppFig3.pdf94KSupplemental Figure 3. Cell differentiation in Notch1Δ/Δ embryonic lungs. CC10, Foxj1, PGP9.5, SpB, SpC and Nkx2.1 in E18.5 Notch1fl/fl and Notch1Δ/Δ lungs were analyzed by real-time PCR.
STEM_1059_sm_SuppFig4.tif424KSupplemental Figure 4. Colocalization of activated NOTCH1 with markers for Clara or ciliated cells in wild-type E18.5 lungs. Immunolocalization of activated NOTCH1 and CC10 (Panel a) or β-tubulin (Panel b) in wild-type E18.5 lungs. Double positive cells (arrow) and single positive cells (triangle).
STEM_1059_sm_SuppFig5.tif470KSupplemental Figure 5. PGP9.5positive cells are not ciliated cells in injured and noninjured lungs. Immunolocalization of PGP9.5 and β - tubulin in uninjured lungs (Panels a & c) and lungs after 3 days of naphthalene injury (Panels b & d). PGP9.5positive (red, arrow) and β - tubulinpositive (green, triangle).
STEM_1059_sm_SuppFig6.tif1086KSupplemental Figure 6. Quantification of cell proliferation after Naphthalene injury. A. Percentage of proliferating cells (Ki67positive) on days 2, 3, 5 and 7 post-injury. Oil was used as injury control. B. Percent proliferating Clara cells (double Ki67positive; CC10positive cells) in airways of control and mutant mice. C. Percent proliferating PGP9.5positive cells (double Phospho-Histone3positive; PGP9.5positive cells) in the airways of mutant and control mice. Cell numbers were determined by manual counting on multiple random fields (n=10).

Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.