Telephone: 1-604-822-7138; Fax: 1-604-822-7815
Article first published online: 15 MAY 2012
Copyright © 2012 AlphaMed Press
Volume 30, Issue 6, pages 1152–1162, June 2012
How to Cite
Lemos, D. R., Paylor, B., Chang, C., Sampaio, A., Underhill, T. M. and Rossi, F. M. V. (2012), Functionally Convergent White Adipogenic Progenitors of Different Lineages Participate in a Diffused System Supporting Tissue Regeneration. STEM CELLS, 30: 1152–1162. doi: 10.1002/stem.1082
Author contributions: D.R.L.: conception and design, collection and/or assembly of data, data analysis and interpretation, manuscript writing, and final approval of manuscript; B.P. and C.C.: collection and/or assembly of data, data analysis and interpretation, and final approval of manuscript; A.S.: conception and design, collection and/or assembly of data, data analysis and interpretation, and final approval of manuscript; M.U.: conception and design, data analysis and interpretation, and final approval of manuscript; F.M.V.R.: conception and design, data analysis and interpretation, manuscript writing, financial support, and final approval of manuscript.
Disclosure of potential conflicts of interest is found at the end of this article.
First published online in STEM CELLSEXPRESS March 13, 2012.
- Issue published online: 15 MAY 2012
- Article first published online: 15 MAY 2012
- Accepted manuscript online: 13 MAR 2012 02:27PM EST
- Manuscript Accepted: 11 FEB 2012
- Manuscript Received: 16 NOV 2011
- CIHR. Grant Number: MOP-82864
- Heart and Stroke Foundation of Canada
- Adipocite progenitor;
- Muscle injury
Pathologies characterized by lipomatous infiltration of craniofacial structures as well as certain forms of lipodystrophies suggest the existence of a distinct adipogenic program in the cephalic region of mammals. Using lineage tracing, we studied the origin of craniofacial adipocytes that accumulate both in cranial fat depots and during ectopic lipomatous infiltration of craniofacial muscles. We found that unlike their counterparts in limb muscle, a significant percentage of cranial adipocytes is derived from the neural crest (NC). In addition, we identified a population of NC-derived Lin−/α7−/CD34+/Sca-1+ fibro/adipogenic progenitors (NC-FAPs) that resides exclusively in the mesenchyme of cephalic fat and muscle. Comparative analysis of the adipogenic potential, impact on metabolism, and contribution to the regenerative response of NC-FAPs and mesoderm-derived FAPs (M-FAPs) suggests that these cells are functionally indistinguishable. While both NC- and M-FAPs express mesenchymal markers and promyogenic cytokines upon damage-induced activation, NC-FAPs additionally express components of the NC developmental program. Furthermore, we show that craniofacial FAP composition changes with age, with young mice containing FAPs that are almost exclusively of NC origin, while NC-FAPs are progressively replaced by M-FAPs as mice age. Based on these results, we propose that in the adult, ontogenetically distinct FAPs form a diffused system reminiscent of the endothelium, which can originate from multiple developmental intermediates to seed all anatomical locations. STEM CELLS2012;30:1152–1162