Regenerative Medicine

Derivation of Mesenchymal Stem Cells from Human Induced Pluripotent Stem Cells Cultured on Synthetic Substrates†‡§

  1. L.G. Villa-Diaz1,
  2. S.E. Brown1,
  3. Y. Liu2,
  4. A.M. Ross3,
  5. J. Lahann3,
  6. J.M. Parent2,4,
  7. P.H. Krebsbach1,¶,*

Article first published online: 15 MAY 2012

DOI: 10.1002/stem.1084

Issue

STEM CELLS

STEM CELLS

Volume 30, Issue 6, pages 1174–1181, June 2012

Additional Information

How to Cite

Villa-Diaz, L.G., Brown, S.E., Liu, Y., Ross, A.M., Lahann, J., Parent, J.M. and Krebsbach, P.H. (2012), Derivation of Mesenchymal Stem Cells from Human Induced Pluripotent Stem Cells Cultured on Synthetic Substrates. STEM CELLS, 30: 1174–1181. doi: 10.1002/stem.1084

Author Information

  1. 1

    Department of Biologic & Materials Sciences, School of Dentistry, University of Michigan, Ann Arbor, Michigan, USA

  2. 2

    Department of Neurology, Medical School, University of Michigan, Ann Arbor, Michigan, USA

  3. 3

    Department of Chemical Engineering, University of Michigan, Ann Arbor, Michigan, USA

  4. 4

    Neurology Service, Veterans Administrator Ann Arbor Healthcare System, University of Michigan, Ann Arbor, Michigan, USA

  1. Telephone: (734)-936-2600; Fax: (734)-647-2110

*Department of Biologic and Materials Sciences, School of Dentistry, University of Michigan, 1011 North University Ave, Ann Arbor, Michigan 48109, USA

  1. †

    Author contributions: L.G.V.-D.: conception and design, collection and/or assembly of data, data analysis and interpretation, manuscript writing, and final approval of manuscript; S.E.B.: collection and/or assembly of data, data analysis and interpretation, and final approval of manuscript; Y.L.: provision of study material, collection and/or assembly of data, data analysis and interpretation, manuscript writing, and final approval of manuscript; A.M.R. and J.L.: provision of study material and final approval of manuscript; J.M.P.: financial support, provision of study material, and final approval of manuscript; P.H.K.: conception and design, financial support, data analysis and interpretation, and final approval of manuscript.

  2. ‡

    Disclosure of potential conflicts of interest is found at the end of this article.

  3. §

    First published online in STEM CELLSEXPRESS March 13, 2012.

  4. Telephone: (734)-936-2600; Fax: (734)-647-2110

Publication History

  1. Issue published online: 15 MAY 2012
  2. Article first published online: 15 MAY 2012
  3. Accepted manuscript online: 13 MAR 2012 02:32PM EST
  4. Manuscript Accepted: 19 FEB 2012
  5. Manuscript Received: 10 AUG 2011

Funded by

  • NIH. Grant Numbers: R01 DE016530, R01 NS065450, F31 DE019760
  • NIDCR T32 Tissue Engineering and Regeneration Training Program

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Keywords:

  • Induced pluripotent stem cells;
  • Mesenchymal stem cells;
  • Bone;
  • Xenogeneic-free culture;
  • Regeneration

Abstract

Human-induced pluripotent stem cells (hiPSCs) may represent an ideal cell source for research and applications in regenerative medicine. However, standard culture conditions that depend on the use of undefined substrates and xenogeneic medium components represent a significant obstacle to clinical translation. Recently, we reported a defined culture system for human embryonic stem cells using a synthetic polymer coating, poly[2-(methacryloyloxy)ethyl dimethyl-(3-sulfopropyl)ammonium hydroxide] (PMEDSAH), in conjunction with xenogeneic-free culture medium. Here, we tested the hypothesis that iPSCs could be maintained in an undifferentiated state in this xeno-free culture system and subsequently be differentiated into mesenchymal stem cells (iPS-MSCs). hiPSCs were cultured on PMEDSAH and differentiated into functional MSCs, as confirmed by expression of characteristic MSC markers (CD166+, CD105+, CD90+,CD73+, CD31−, CD34−, and CD45−) and their ability to differentiate in vitro into adipogenic, chondrogenic, and osteoblastic lineages. To demonstrate the potential of iPS-MSCs to regenerate bone in vivo, the newly derived cells were induced to osteoblast differentiation for 4 days and transplanted into calvaria defects in immunocompromised mice for 8 weeks. MicroCT and histologic analyses demonstrated de novo bone formation in the calvaria defects for animals treated with iPS-MSCs but not for the control group. Moreover, positive staining for human nuclear antigen and human mitochondria monoclonal antibodies confirmed the participation of the transplanted hiPS-MSCs in the regenerated bone. These results demonstrate that hiPSCs cultured in a xeno-free system have the capability to differentiate into functional MSCs with the ability to form bone in vivo. STEM CELLS2012;30:1174–1181

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