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Additional Supporting Information may be found in the online version of this article.

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SC_11-1027_sm_SupplData.pdf134KSupplementary Data
SC_11-1027_sm_SupplFigure1.pdf237KSupplemental figure 1. Immunocytological assessment of cardiomyogenic differentiation of genetically modified adult AT hMSCs after 10 days of co-culture with nrCMCs. (A-B) Upon co-culture with nrCMCs, Cx43[UPWARDS ARROW] adult AT hMSCs nor control adult AT hMSCs stained positive for α-actinin. In panel A, please note the Cx43 (indicated as Cx) plaques at the Cx43[UPWARDS ARROW] adult AT hMSCs-nrCMC interface.
SC_11-1027_sm_SupplFigure2.pdf748KSupplemental figure 2. Immunocytological assessment of cardiomyogenic differentiation of genetically modified adult AT hMSCs and fetal AM hMSCs after 10 days of co-culture with nrCMCs. (A1) Upon co-culture with nrCMCs, a fraction of the GFP-positive control fetal AM hMSCs became positive for the cardiac transcription factor Nkx2.5. (A2-A3, B1- B2) GFP-labeled Cx43[DOWNWARDS ARROW](1) fetal AM hMSCs, Cx43[DOWNWARDS ARROW](2) fetal AM hMSCs, Cx43[UPWARDS ARROW] adult AT hMSCs and control adult AT hMSCs in co-culture with nrCMCs did not stain positive for Nkx2.5.
SC_11-1027_sm_SupplFigure3.pdf768KSupplemental figure 3. Immunocytological assessment of cardiomyogenic differentiation of genetically modified adult AT hMSCs and fetal AM hMSCs after 10 days of co-culture with nrCMCs. (A1) Upon co-culture with nrCMCs, a fraction of the GFP-positive control fetal AM hMSCs became positive for the cardiac transcription factor GATA4. (A2-A3, B1- B2) GFP-labeled Cx43[DOWNWARDS ARROW](1) fetal AM hMSCs, Cx43[DOWNWARDS ARROW](2) fetal AM hMSCs, Cx43[UPWARDS ARROW] adult AT hMSCs and control adult AT hMSCs in co-culture with nrCMCs did not stain positive for GATA4.
SC_11-1027_sm_SupplFigure4.pdf850KSupplemental figure 4. Investigation by immunocytological staining of cardiomyogenic differentiation of fetal AM hMSCs in co-culture with nrCMCs after rescue of Cx43 knockdown by Cx45 overexpression. In the presence of nrCMCs, a fraction of the GFPpositive Cx43[DOWNWARDS ARROW](1)+Cx45[UPWARDS ARROW] fetal AM hMSCs (A1 and B1) expressed Nkx2.5 and GATA4, while Cx43[DOWNWARDS ARROW](1)+eGFP fetal AM hMSCs (A2 and B2) did not.

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