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Tissue-Specific Stem Cells
Article first published online: 15 MAY 2012
Copyright © 2012 AlphaMed Press
Volume 30, Issue 6, pages 1265–1276, June 2012
How to Cite
Ziegler, A. N., Schneider, J. S., Qin, M., Tyler, W. A., Pintar, J. E., Fraidenraich, D., Wood, T. L. and Levison, S. W. (2012), IGF-II Promotes Stemness of Neural Restricted Precursors. STEM CELLS, 30: 1265–1276. doi: 10.1002/stem.1095
Author contributions: A.N.Z.: conception and design, financial support, collection of data, data analysis and interpretation, manuscript writing, and final approval of manuscript; J.S.S. and M.Q.: collection of data, data analysis and interpretation, manuscript writing, and final approval of manuscript; W.A.T.: data analysis and interpretation, manuscript writing, and final approval of manuscript; J.E.P., D.F., T.L.W., and S.W.L.: administrative support, financial support, conception and design, manuscript writing, and final approval of manuscript. T.L.W. and S.W.L. are cosenior authors.
Disclosure of potential conflicts of interest is found at the end of this article.
First published online in STEM CELLSEXPRESS March 29, 2012.
- Issue published online: 15 MAY 2012
- Article first published online: 15 MAY 2012
- Accepted manuscript online: 29 MAR 2012 09:12AM EST
- Manuscript Accepted: 1 MAR 2012
- Manuscript Received: 1 FEB 2011
- R21HL094905 awarded
- NJMSm. Grant Number: F31NS065607
- LeDucq Foundation
Additional Supporting Information may be found in the online version of this article.
|SC_11-0119_sm_supplFigure1.pdf||194K||Figure S1: IGF-II restores neurosphere number while a combination of both IGF-I and IGF-II restores normal growth. A,B,C,D. Secondary neurospheres were dissociated and grown in media with 4.4 μM or 4.4 nM insulin with EGF at 20 ng/mL. A,B. Medium was supplemented with 2 doses of IGF-I, KD (2 nM) and 8x KD (15 nM). C,D. Medium was supplemented with 2 doses of IGF-II, KD (15 nM) and 8x KD (30 nM). Cells were cultured 6 DIV to enable sphere formation. Sphere volume (A,C) and number (B,D) was quantified with results expressed as means ± SEM. Significant difference determined by ANOVA followed by TUKEY's post hoc p<0.05, a,b,c,d denotes groups.|
|SC_11-0119_sm_supplTable1.pdf||10K||Supplementary Table 1|
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