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SC-11-0973_sm_SupplFig1.pdf467KFig. S1. Experimental design. (A) Neural precursor cells were transplanted into the left ischemic striatum after a 45-min stroke. LV: lateral ventricle. (B) Timeline of experimental in vivo paradigm. Time points given in hours (h) and days (d) refer to induction of stroke. BBB: blood-brain-barrier; MMP-9: matrix metalloproteinase 9; TBARS: thiobarbituric acid reactive substances; IHC: immunohistochemistry; ELISA: enzyme-linked immunosorbent assay.
SC-11-0973_sm_SupplFig2.pdf57KFig. S2. Detection of TAT-Hsp70 in cultivated NPCs in vitro. Cultivated neural precursor cells (NPCs) from passage 4 were incubated for 4 h with/without TAT-Hsp70 or TAT-HA (250 nM). Thereafter, cells were washed and lysed. Lysates were used for preparation of slot blots for detection of the hemagglutinin (HA) sequence of the recombinant proteins, which is not present in mammalian cells under physiological conditions. TAT-Hsp70 served as positive control, whereas actin served as loading control.
SC-11-0973_sm_SupplFig3.pdf772KFig. S3. Differentiation analysis of transplanted neural precursor cells. State of differentiation of grafted GFP+ neural precursor cells (NPCs) was analyzed using antibodies against the astroglial marker GFAP (A-D), the oligodendroglial marker CNPase (E-H), the neural marker nestin (I-L), and the immature neuronal marker Dcx (M-P). All photos are exemplarily taken on day 56 from animals that had received intracerebral transplantation of TAT-Hsp70-transduced NPCs. Arrows indicate co-localization of grafted NPCs with the marker in question. Scale bar: 20 μm.
SC-11-0973_sm_SupplMaterials.pdf10KSupplementary Data

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