Telephone: +49-201-723-83133; Fax: +49-201-723-1660
Translational and Clinical Research
Article first published online: 15 MAY 2012
Copyright © 2012 AlphaMed Press
Volume 30, Issue 6, pages 1297–1310, June 2012
How to Cite
Doeppner, T. R., Ewert, T. A. S., Tönges, L., Herz, J., Zechariah, A., ElAli, A., Ludwig, A.-K., Giebel, B., Nagel, F., Dietz, G. P. H., Weise, J., Hermann, D. M. and Bähr, M. (2012), Transduction of Neural Precursor Cells with TAT-Heat Shock Protein 70 Chaperone: Therapeutic Potential Against Ischemic Stroke after Intrastriatal and Systemic Transplantation. STEM CELLS, 30: 1297–1310. doi: 10.1002/stem.1098
Author contributions: T.R.D.: conception and design, collection and/or assembly of data, data analysis and interpretation, manuscript writing, and final approval of manuscript; T.A.E.: collection and/or assembly of data, provision of study material or patients, and final approval of manuscript; L.T. and F.N.: provision of study material or patients and final approval of manuscript; J.H., A.Z., A.E., and A.-K.L.: collection and/or assembly of data and final approval of manuscript; B.G. and G.P.D.: manuscript writing and final approval of manuscript; J.W.: conception and design, financial support, and final approval of manuscript; D.M.H.: administrative support, manuscript writing, and final approval of manuscript; M.B.: financial support, administrative support, and final approval of manuscript.
Disclosure of potential conflicts of interest is found at the end of this article.
First published online in STEM CELLSEXPRESS March 30, 2012.
- Issue published online: 15 MAY 2012
- Article first published online: 15 MAY 2012
- Accepted manuscript online: 30 MAR 2012 10:21AM EST
- Manuscript Accepted: 16 MAR 2012
- Manuscript Received: 6 OCT 2011
Additional Supporting Information may be found in the online version of this article.
|SC-11-0973_sm_SupplFig1.pdf||467K||Fig. S1. Experimental design. (A) Neural precursor cells were transplanted into the left ischemic striatum after a 45-min stroke. LV: lateral ventricle. (B) Timeline of experimental in vivo paradigm. Time points given in hours (h) and days (d) refer to induction of stroke. BBB: blood-brain-barrier; MMP-9: matrix metalloproteinase 9; TBARS: thiobarbituric acid reactive substances; IHC: immunohistochemistry; ELISA: enzyme-linked immunosorbent assay.|
|SC-11-0973_sm_SupplFig2.pdf||57K||Fig. S2. Detection of TAT-Hsp70 in cultivated NPCs in vitro. Cultivated neural precursor cells (NPCs) from passage 4 were incubated for 4 h with/without TAT-Hsp70 or TAT-HA (250 nM). Thereafter, cells were washed and lysed. Lysates were used for preparation of slot blots for detection of the hemagglutinin (HA) sequence of the recombinant proteins, which is not present in mammalian cells under physiological conditions. TAT-Hsp70 served as positive control, whereas actin served as loading control.|
|SC-11-0973_sm_SupplFig3.pdf||772K||Fig. S3. Differentiation analysis of transplanted neural precursor cells. State of differentiation of grafted GFP+ neural precursor cells (NPCs) was analyzed using antibodies against the astroglial marker GFAP (A-D), the oligodendroglial marker CNPase (E-H), the neural marker nestin (I-L), and the immature neuronal marker Dcx (M-P). All photos are exemplarily taken on day 56 from animals that had received intracerebral transplantation of TAT-Hsp70-transduced NPCs. Arrows indicate co-localization of grafted NPCs with the marker in question. Scale bar: 20 μm.|
Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.