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Embryonic Stem Cells/Induced Pluripotent Stem Cells
Article first published online: 24 JUL 2012
Copyright © 2012 AlphaMed Press
Volume 30, Issue 8, pages 1611–1623, August 2012
How to Cite
Koyano-Nakagawa, N., Kweon, J., Iacovino, M., Shi, X., Rasmussen, T. L., Borges, L., Zirbes, K. M., Li, T., Perlingeiro, R. C. R., Kyba, M. and Garry, D. J. (2012), Etv2 Is Expressed in the Yolk Sac Hematopoietic and Endothelial Progenitors and Regulates Lmo2 Gene Expression. STEM CELLS, 30: 1611–1623. doi: 10.1002/stem.1131
Author contributions: N.K.-N.: conception and design, financial support, collection and assembly of data, data analysis and interpretation, and manuscript writing; J.K. and M.I.: conception and design, collection and assembly of data, data analysis and interpretation, and manuscript writing; X.S., T.L.R., and L.B.: conception and design, collection and assembly of data, and data analysis and interpretation; K.M.Z.: collection and assembly of data and data analysis; T.L.: data analysis and interpretation; R.C.R.P. and M.K.: provision of study material, data interpretation, and financial support; D.J.G.: conception and design, financial support, data interpretation, manuscript writing, and final approval of manuscript. N.K.-N and J.K contributed equally to this article.
Disclosure of potential conflicts of interest is found at the end of this article.
First published online in STEM CELLSEXPRESS Month 00, 2012.
- Issue published online: 24 JUL 2012
- Article first published online: 24 JUL 2012
- Accepted manuscript online: 24 MAY 2012 11:15AM EST
- Manuscript Accepted: 11 MAY 2012
- Manuscript Received: 26 SEP 2011
- National Institutes of Health. Grant Numbers: U01 HL100407, P01 GM081627, R01 HL085840, R01 HL085729
- American Heart Association
- Jon Holden DeHaan Foundation. Grant Number: 0970499
- Grant-in-Aid of Research, Artistry, and Scholarship, University of Minnesota
- Established Investigator of the American Heart Association
- Etv2 knockout;
- Endothelial development
During embryogenesis, the endothelial and the hematopoietic lineages first appear during gastrulation in the blood island of the yolk sac. We have previously reported that an Ets variant gene 2 (Etv2/ER71) mutant embryo lacks hematopoietic and endothelial lineages; however, the precise roles of Etv2 in yolk sac development remains unclear. In this study, we define the role of Etv2 in yolk sac blood island development using the Etv2 mutant and a novel Etv2-EYFP reporter transgenic line. Both the hematopoietic and the endothelial lineages are absent in the Etv2 mutant yolk sac. In the Etv2-EYFP transgenic mouse, the EYFP reporter is activated in the nascent mesoderm, expressed in the endothelial and blood progenitors, and in the Tie2+, c-kit+, and CD41+ hematopoietic population. The hematopoietic activity in the E7.75 yolk sac was exclusively localized to the Etv2-EYFP+ population. In the Etv2 mutant yolk sac, Tie2+ cells are present but do not express hematopoietic or endothelial markers. In addition, these cells do not form hematopoietic colonies, indicating an essential role of Etv2 in the specification of the hematopoietic lineage. Forced overexpression of Etv2 during embryoid body differentiation induces the hematopoietic and the endothelial lineages, and transcriptional profiling in this context identifies Lmo2 as a downstream target. Using electrophoretic mobility shift assay, chromatin immunoprecipitation, transcriptional assays, and mutagenesis, we demonstrate that Etv2 binds to the Lmo2 enhancer and transactivates its expression. Collectively, our studies demonstrate that Etv2 is expressed during and required for yolk sac hematoendothelial development, and that Lmo2 is one of the downstream targets of Etv2. STEM CELLS2012;30:1611–1623