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SC_12-0214_sm_supplFigure1.tif672KSUPPLEMENTARY FIGURE 1: Study design. C57BL/6 adult male mice received citrate buffer (Normal) or 40 mg/kg/day STZ for 5 days (T1DM). Twenty-five days after the first STZ dose, diabetic mice received via the tail vein the vehicle (T1DM) or 5×105 MSCs (T1DM+MSC). Blood glucose levels were determined every three days. Seven and 65 days post-transplantation, samples were taken for analysis (*).
SC_12-0214_sm_supplFigure2.tif2659KSUPPLEMENTARY FIGURE 2: Gating strategy used for the determination of autoaggressive T cells. Lymphocytes were gated base on FSC and SCC (R1 in A). Then, according to the no expression of CD45R (R2 in B), and no expression of CD19 and CD8 but expression of CD4 (R3 in C). CD45R−/CD19−/CD8−/CD4lo cells were analyzed according to the expression of CD3 and CD40. Events in R4 (CD45R−/CD19−/CD8−/CD4lo/CD3lo/CD40+) correspond to auto-aggressive T cells (D).
SC_12-0214_sm_supplFigure3.tif1891KSUPPLEMENTARY FIGURE 3: Gating strategy used for the determination of regulatory T cells. Lymphocytes were gated base on FSC and SCC (R1 in A). Then, according to the expression of CD4 (R2 in B), and CD25 and foxp3. Events in R3 (CD4+/CD25+/foxp3+) correspond to regulatory T cells (C).
SC_12-0214_sm_supplFigure4.tif2227KSUPPLEMENTARY FIGURE 4: Gating strategy used for the assessment of ex vivo activation of T lymphocytes. Lymphocytes were gated base on FSC and SCC (R1 in A). Then, according to the expression of CD4 (R2 in B), and IL2, IFNgamma or TNFalpha. Events in R3 (CD4+/IL2+), R4 (CD4+/IFNgamma+) and R5 (CD4+/TNFalpha+) correspond to activated T lymphocytes (C, D and E, respectively).
SC_12-0214_sm_supplTable1.tif1605KSUPPLEMENTARY TABLE 1: Primer and amplicon characteristics.

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