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Article first published online: 24 JUL 2012
Copyright © 2012 AlphaMed Press
Volume 30, Issue 8, pages 1664–1674, August 2012
How to Cite
Ezquer, F., Ezquer, M., Contador, D., Ricca, M., Simon, V. and Conget, P. (2012), The Antidiabetic Effect of Mesenchymal Stem Cells Is Unrelated to Their Transdifferentiation Potential But to Their Capability to Restore Th1/Th2 Balance and to Modify the Pancreatic Microenvironment. STEM CELLS, 30: 1664–1674. doi: 10.1002/stem.1132
Author contributions: F.E.: conception and design, financial support, collection of data, data analysis, manuscript writing, and final approval of manuscript; M.E.: conception and design and data analysis; D.C. and M.R.: collection of data; V.S.: collection of data and data analysis; P.C.: conception and design, financial support, data analysis, manuscript writing, and final approval of manuscript.
Disclosure of potential conflicts of interest is found at the end of this article.
First published online in STEM CELLSEXPRESS May 29, 2012.
- Issue published online: 24 JUL 2012
- Article first published online: 24 JUL 2012
- Accepted manuscript online: 29 MAY 2012 08:43AM EST
- Manuscript Accepted: 4 MAY 2012
- Manuscript Received: 3 MAR 2012
- FONDECYT. Grant Number: #11085033
Additional Supporting Information may be found in the online version of this article.
|SC_12-0214_sm_supplFigure1.tif||672K||SUPPLEMENTARY FIGURE 1: Study design. C57BL/6 adult male mice received citrate buffer (Normal) or 40 mg/kg/day STZ for 5 days (T1DM). Twenty-five days after the first STZ dose, diabetic mice received via the tail vein the vehicle (T1DM) or 5×105 MSCs (T1DM+MSC). Blood glucose levels were determined every three days. Seven and 65 days post-transplantation, samples were taken for analysis (*).|
|SC_12-0214_sm_supplFigure2.tif||2659K||SUPPLEMENTARY FIGURE 2: Gating strategy used for the determination of autoaggressive T cells. Lymphocytes were gated base on FSC and SCC (R1 in A). Then, according to the no expression of CD45R (R2 in B), and no expression of CD19 and CD8 but expression of CD4 (R3 in C). CD45R−/CD19−/CD8−/CD4lo cells were analyzed according to the expression of CD3 and CD40. Events in R4 (CD45R−/CD19−/CD8−/CD4lo/CD3lo/CD40+) correspond to auto-aggressive T cells (D).|
|SC_12-0214_sm_supplFigure3.tif||1891K||SUPPLEMENTARY FIGURE 3: Gating strategy used for the determination of regulatory T cells. Lymphocytes were gated base on FSC and SCC (R1 in A). Then, according to the expression of CD4 (R2 in B), and CD25 and foxp3. Events in R3 (CD4+/CD25+/foxp3+) correspond to regulatory T cells (C).|
|SC_12-0214_sm_supplFigure4.tif||2227K||SUPPLEMENTARY FIGURE 4: Gating strategy used for the assessment of ex vivo activation of T lymphocytes. Lymphocytes were gated base on FSC and SCC (R1 in A). Then, according to the expression of CD4 (R2 in B), and IL2, IFNgamma or TNFalpha. Events in R3 (CD4+/IL2+), R4 (CD4+/IFNgamma+) and R5 (CD4+/TNFalpha+) correspond to activated T lymphocytes (C, D and E, respectively).|
|SC_12-0214_sm_supplTable1.tif||1605K||SUPPLEMENTARY TABLE 1: Primer and amplicon characteristics.|
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