Roles of Planar Cell Polarity Signaling in Maturation of Neuronal Precursor Cells in the Postnatal Mouse Olfactory Bulb§

Authors


  • Author contributions: Y.H. and M. Sawada: conception and design, collection and assembly of data, data analysis and interpretation, and manuscript writing; Y.S.K.: conception and design, collection and assembly of data, data analysis and interpretation, and provision of study material or patients; S.H.: collection and assembly of data and data analysis and interpretation; M.Sakaguchi and O.Y.: conception and design and collection and assembly of data; T.O.: conception and design, provision of study material or patients, financial support, and administrative support; H.O.: conception and design, data analysis and interpretation, financial support, and administrative support; K.S.: conception and design, data analysis and interpretation, financial support, manuscript writing, and administrative support.

  • Disclosure of potential conflicts of interest is found at the end of this article.

  • §

    First published online in STEM CELLSEXPRESS May 24, 2012.

Abstract

Neuronal precursor cells generated by stem cells in the subventricular zone (SVZ) migrate and differentiate into mature interneurons in the olfactory bulb (OB). The mechanisms responsible for the dynamic morphological changes in cells during this process are largely unknown. Wnt/planar cell polarity (PCP) signaling regulates various developmental events, including neuronal migration and neurite formation. Here, we studied the function of two components of the PCP pathway, Dishevelled2 and Van Gogh like-2, in the newborn neurons in the postnatal mouse OB. Electroporation- or lentivirus-mediated introduction of vectors carrying a knockdown or dominant-negative construct of these genes into the SVZ altered the distribution and dendrite formation of newborn neurons in the OB, suggesting that PCP signaling is involved in regulating the maturation of new neurons in the OB. STEM CELLS 2012;30:1726-1733

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