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Additional Supporting Information may be found in the online version of this article.

FilenameFormatSizeDescription
SC_12-0282_sm_supplFigure1.tif1080KFigure S1: Log2 transformations of cRPKM. A) Scatter plots of the log2 transformed data of one tissue vs. the other (STEM: stem cell, PROG: stem cell progeny, IRRAD: differentiated tissue compartments ). B) Histograms of the log2 transformed data.
SC_12-0282_sm_supplFigure2.tif238KFigure S2: Distribution of the ranking (log-fold change) used for GSEA analysis for the comparison between stem cell and stem cell progeny. A) Distribution of the ranking used for GSEA and created from the log-fold change values. B) Distribution of the log-fold change values calculated for the comparison between stem cell and stem cell progeny.
SC_12-0282_sm_supplFigure3.tif3302KFigure S3: Timecourse of piwi-1 loss during 9 days post-RNAi of NOP2. Earliest time point (day 3 post-RNAi) and least severe is on the upper left. Most severe and latest time point (day 9) is on the lower right.
SC_12-0282_sm_supplFigure4.tif2220KFigure S4: Timecourse of piwi-1 loss during 9 days post-RNAi of SMD1. Earliest time point (day 3 post-RNAi) and least severe is on the upper left. Most severe and latest time point (day 9) is on the lower right.
SC_12-0282_sm_supplFigure5.tif2419KFigure S5: Timecourse of piwi-1 loss during 9 days post-RNAi of PHB. Earliest time point (day 3 post-RNAi) and least severe is on the upper left. Most severe and latest time point (day 9) is on the lower right.
SC_12-0282_sm_supplFigure6.tif1003KFigure S6: Timecourse of piwi-1 loss during 6 days post-RNAi of PSMC4. Earliest time point (day 3 post-RNAi) and least severe is on the upper left. Most severe and latest time point (day 6) is on the lower right.
SC_12-0282_sm_supplFigure7.tif979KFigure S7: Timecourse of piwi-1 loss during 6 days post-RNAi of RUVB2. Earliest time point (day 3 post-RNAi) and least severe is on the upper left. Most severe and latest time point (day 6) is on the lower right.
SC_12-0282_sm_supplFigure8.pdf331KFigure S8: WT expression patterns of identified genes from Figs. 5-7 that gave whole-mount ISH results. All genes shown have mesenchymal staining patterns, which is similar to the stem cell marker piwi-1 in Fig. 1A.
SC_12-0282_sm_supplFigure9.tif2129KFigure S9: In support of piwi-1 loss correlating to stem cell loss, we assayed the stem cell marker Histone 2A (H2A) during the knockdown of several genes used in Figs. 5-7. Similar to piwi-1 loss, H2A is lost on the same timecourse. All panels show experimental day 12 except for PSMC4, which was day 10.
SC_12-0282_sm_supplTable1.xls2480KSupplementary Table 1
SC_12-0282_sm_supplTable2.xls43KSupplementary Table 2
SC_12-0282_sm_supplTable3.xls1197KSupplementary Table 3
SC_12-0282_sm_supplTable4.xls33KSupplementary Table 4
SC_12-0282_sm_supplTable5.xls192KSupplementary Table 5
SC_12-0282_sm_supplTable6.xls37KSupplementary Table 6

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