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Additional Supporting Information may be found in the online version of this article.

FilenameFormatSizeDescription
SC_12-0081_sm_supplTable1.pdf12KSupplementary Table 1
SC_12-0081_sm_supplTable2.pdf60KSupplementary Table 2
SC_12-0081_sm_supplFigure1.jpg135KSupplementary Figure 1. E-cadherin and N-cadherin promote STAT3 phosphorylation and Klf4 and Nanog expression in mES cells. (A): Immunofluorescent and flow cytometry analysis of Klf4 and Nanog protein expression in Ecad−/− mES cells cultured in FBScontaining medium or LIF/BMP medium. (B): Immunofluorescent and flow cytometry analysis of Klf4 and Nanog protein expression in Ecad−/− mES cells transfected with FL-Ecad or the control plasmid, pCMV. (C): Western blot analysis for pSTAT3 in wtD3 mES cells treated with E/N+ or the control peptide, E/N− for 3 hours in FBS-containing medium. All scale bars: 100μm.
SC_12-0081_sm_supplFigure2.jpg136KSupplementary Figure 2. Confirmation of results from single cells. (A): RT-PCR analysis of N-cadherin and Klf4 transcripts in wtD3 and Ecad−/− mES cells cultured in FBS-containing medium and LIF/BMP medium. (B): qPCR analysis of Klf4 and Nanog transcripts in wtD3 and Ecad−/− mES cells in wtD3 and Ecad−/− mES cells cultured in FBS-containing medium and LIF/BMP medium. (C): Flow cytometry and immunofluorescent analysis of Oct4 expression (green) wtD3 and Ecad−/− mES cells cultured in FBS-containing medium and LIF/BMP medium and treated with the Alk inhibitor SB431542. Nuclei were stained with DAPI (blue). Scale bars: 100μm. (D): Western blot analysis of pSTAT3, STAT3, (E): Nanog and α-tubulin protein expression in wtD3 and Ecad−/− mES cells cultured in FBS-containing medium or LIF/BMP medium.
SC_12-0081_sm_supplFigure3.jpg152KSupplementary Figure 3. Ecad−/− mES cells exhibit N-cadherin-mediated STAT3 phosphorylation and upregulation of Klf4 and Nanog when cultured in 2i medium. (A): Phase contrast images of wtD3 and Ecad−/− mES cells grown in 2i medium. (B): Immuostaining for Oct4, Nanog, Klf4 and N-cadherin in Ecad−/− mES cells grown in 2i medium. (C): Phase contrast images of Ecad−/− mES cells grown in 2i medium and exposed to the E/N+ peptide inhibitor or its control, E/N−. (D): Western blot analysis of STAT3 phosphoryation in wtD3 and Ecad−/− mES cells grown in FBS-containing medium and 2i medium with the E- and Ncadherin inhibitory peptide E/N+, the control peptide E/N− or untreated (2i). (E): RT-PCR analysis of Klf4, Nanog and N-cadherin in Ecad−/− mES cells grown in 2i medium and exposed to the E/N+ peptide, the control peptide, E/N−, or untreated (2i). (F): QPCR analysis of Klf4 and Nanog transcript expression in Ecad−/− mES cells grown in 2i medium and exposed to the E/N+ peptide, the control peptide, E/N−, or untreated (2i). All scale bars: 100μm.

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