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Additional Supporting Information may be found in the online version of this article.

FilenameFormatSizeDescription
SC_11-1242_sm_supplFigure1.tif1651KSupplementary Figure 1 The ectopic expression of miR-138 detected by qPCR and Northern blot. To check the overexpression level of miR-138, we loaded 1 μg miR-138 DNA oligos as positive control, while we had to decrease the amount of loaded miR-138 DNA oligos to 1 ng as positive control to ensure the exposure time of endogenous miR-138. **P value < 0.01. The error bar represents the standard deviation (SD) of three independent experiments.
SC_11-1242_sm_supplFigure2.tif1539KSupplementary Figure 2 Relative mRNA level of p53 in the reprogrammed fibroblasts transduced with OSKM, OSKM+miR-138 and OSKM+miR-138 sponge. **P value < 0.01; *P value < 0.05. The error bar represents the standard deviation (SD) of three independent experiments. The protein level of p53 is to check miR-138 sponge effects. GFP infection serves as a negative control. GAPDH serves as a loading control.
SC_11-1242_sm_supplFigure3.pdf1283KSupplementary Figure 3 (A): The colonies of 138-2-7-iPS cells, mES14.1 cells and 3-2-1-iPS cells show strong immunoreactivity for pluripotency-associated transcription factors (Oct4 and Nanog) and surface marker (SSEA-1). (B): Embryoid bodies of 138-2-7-iPS cells, mES14.1 cells and 3-2-1-iPS cells on day 7 of differentiation, subjected to immunofluorescence for endoderm (AFP), mesoderm (Actin and GATA4) and ectoderm (GFAP and Tuj1). (C): The various tissues were present in teratomas derived from 138-2-7-iPS cells and mES14.1 cells by H&E staining. 138-2-7-panel from left to right: arrowheads, epithelium (endoderm), cartilage (mesoderm) and neural tube (ectoderm). mES14.1-panel from left to right: arrowheads, glandular (endoderm), skeletal muscle tissue (mesoderm) and neural tube (ectoderm).
SC_11-1242_sm_supplFigure4.tif1421KSupplementary Figure 4 The expression level of EZH2 in the MEFs and during iPS induction. *P value < 0.05. The error bar represents the standard deviation (SD) of three independent experiments.
SC_11-1242_sm_supplFigure5.tif1249KSupplementary Figure 5 The protein level of p53 is to confirm p53 overexpression. GFP infection serves as a negative control. GAPDH serves as a loading control.
SC_11-1242_sm_supplTable1.pdf25KSupplementary Table 1
SC_11-1242_sm_supplTable2.pdf73KSupplementary Table 2
SC_11-1242_sm_supplTable3.pdf25KSupplementary Table 3
SC_11-1242_sm_supplTable4.pdf9KSupplementary Table 4
SC_11-1242_sm_supplTable5.xls1396KSupplementary Table 5

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