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Embryonic Stem Cells/Induced Pluripotent Stem Cells
Article first published online: 24 JUL 2012
Copyright © 2012 AlphaMed Press
Volume 30, Issue 8, pages 1645–1654, August 2012
How to Cite
Ye, D., Wang, G., Liu, Y., Huang, W., Wu, M., Zhu, S., Jia, W., Deng, A.-M., Liu, H. and Kang, J. (2012), MiR-138 Promotes Induced Pluripotent Stem Cell Generation Through the Regulation of the p53 Signaling. STEM CELLS, 30: 1645–1654. doi: 10.1002/stem.1149
Author contributions: D.Y.: conception and design, collection and/or assembly of data, data analysis and interpretation, and manuscript writing; G.W.: conception and design, data analysis and interpretation, and manuscript writing; Y.L., W.H., and M.W.: collection and/or assembly of data; S.Z. and W.J.: data analysis and interpretation and manuscript writing; A.D. and H.L.: data analysis and interpretation; J.K.: conception and design, data analysis and interpretation, financial support, manuscript writing, and final approval of manuscript.
Disclosure of potential conflicts of interest is found at the end of this article.
First published online in STEM CELLSEXPRESS June 13, 2012.
- Issue published online: 24 JUL 2012
- Article first published online: 24 JUL 2012
- Accepted manuscript online: 13 JUN 2012 02:40PM EST
- Manuscript Accepted: 29 MAY 2012
- Manuscript Received: 31 DEC 2011
- Ministry of Science and Technology. Grant Numbers: 2011CB965100, 2010CB944900, 2010CB945000, 2011CBA01100, 2011DFA30480
- National Natural Science Foundation of China. Grant Numbers: 90919028, 31071306, 31000378, 31101061, 31171432, 30971451, 81170499
- Science and Technology Commission of Shanghai Municipality. Grant Numbers: 11ZR1438500, 11XD1405300, IRT1168, 20110072110039
- Ministry of Education, China
- Fundamental Research Funds for the Central Universities
Vol. 31, Issue 11, 2585–2586, Article first published online: 15 NOV 2013
Additional Supporting Information may be found in the online version of this article.
|SC_11-1242_sm_supplFigure1.tif||1651K||Supplementary Figure 1 The ectopic expression of miR-138 detected by qPCR and Northern blot. To check the overexpression level of miR-138, we loaded 1 μg miR-138 DNA oligos as positive control, while we had to decrease the amount of loaded miR-138 DNA oligos to 1 ng as positive control to ensure the exposure time of endogenous miR-138. **P value < 0.01. The error bar represents the standard deviation (SD) of three independent experiments.|
|SC_11-1242_sm_supplFigure2.tif||1539K||Supplementary Figure 2 Relative mRNA level of p53 in the reprogrammed fibroblasts transduced with OSKM, OSKM+miR-138 and OSKM+miR-138 sponge. **P value < 0.01; *P value < 0.05. The error bar represents the standard deviation (SD) of three independent experiments. The protein level of p53 is to check miR-138 sponge effects. GFP infection serves as a negative control. GAPDH serves as a loading control.|
|SC_11-1242_sm_supplFigure3.pdf||1283K||Supplementary Figure 3 (A): The colonies of 138-2-7-iPS cells, mES14.1 cells and 3-2-1-iPS cells show strong immunoreactivity for pluripotency-associated transcription factors (Oct4 and Nanog) and surface marker (SSEA-1). (B): Embryoid bodies of 138-2-7-iPS cells, mES14.1 cells and 3-2-1-iPS cells on day 7 of differentiation, subjected to immunofluorescence for endoderm (AFP), mesoderm (Actin and GATA4) and ectoderm (GFAP and Tuj1). (C): The various tissues were present in teratomas derived from 138-2-7-iPS cells and mES14.1 cells by H&E staining. 138-2-7-panel from left to right: arrowheads, epithelium (endoderm), cartilage (mesoderm) and neural tube (ectoderm). mES14.1-panel from left to right: arrowheads, glandular (endoderm), skeletal muscle tissue (mesoderm) and neural tube (ectoderm).|
|SC_11-1242_sm_supplFigure4.tif||1421K||Supplementary Figure 4 The expression level of EZH2 in the MEFs and during iPS induction. *P value < 0.05. The error bar represents the standard deviation (SD) of three independent experiments.|
|SC_11-1242_sm_supplFigure5.tif||1249K||Supplementary Figure 5 The protein level of p53 is to confirm p53 overexpression. GFP infection serves as a negative control. GAPDH serves as a loading control.|
|SC_11-1242_sm_supplTable1.pdf||25K||Supplementary Table 1|
|SC_11-1242_sm_supplTable2.pdf||73K||Supplementary Table 2|
|SC_11-1242_sm_supplTable3.pdf||25K||Supplementary Table 3|
|SC_11-1242_sm_supplTable4.pdf||9K||Supplementary Table 4|
|SC_11-1242_sm_supplTable5.xls||1396K||Supplementary Table 5|
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